HIGH-MOBILITY GROUP PROTEIN HMG-I LOCALIZES TO G/Q-BAND AND C-BAND OF HUMAN AND MOUSE CHROMOSOMES
HIGH-MOBILITY GROUP PROTEIN HMG-I LOCALIZES TO G/Q-BAND AND C-BAND OF HUMAN AND MOUSE CHROMOSOMES
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DOI：
10.1083/jcb.109.5.1975
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发表时间：
1989-11-01
影响因子：
7.8
通讯作者：
REEVES, R
中科院分区：
文献类型：
--
作者：
DISNEY, JE;JOHNSON, KR;REEVES, R
哺乳动物中期染色体经短暂的蛋白酶消化后，用姬姆萨染料染色，可通过其特征性带型进行鉴定。已知产生的G带含有富含A/T残基的DNA区域，并且是人类染色体中长散布重复序列的L1（或Kpn 1）家族的主要位置。在这里，我们报告说，抗体提出了对一个高度纯化和生化良好的特点非组蛋白“高迁移率组”蛋白，HMG-I，专门本地化这种蛋白质的G带在哺乳动物中期染色体。在某些染色体高度浓缩的制备物中，HMG-1似乎也位于哺乳动物染色体的着丝粒和/或端粒区域。据我们所知，这是第一个充分表征的哺乳动物蛋白质，主要定位于染色体的G带区域。
Mammalian metaphase chromosomes can be identified by their characteristic banding pattern when stained with Giemsa dye after brief proteolytic digestion. The resulting G-bands are known to contain regions of DNA enriched in A/T residues and to be the principal location for the L1 (or Kpn 1) family of long interspersed repetitive sequences in human chromosomes. Here we report that antibodies raised against a highly purified and biochemically well characterized nonhistone "High-Mobility Group" protein, HMG-I, specifically localize this protein to the G-bands in mammalian metaphase chromosomes. In some preparations in which chromosomes are highly condensed, HMG-I appears to be located at the centromere and/or telomere regions of mammalian chromosomes as well. To our knowledge, this is the first well-characterized mammalian protein that localizes primarily to G-band regions of chromosomes.