Translation in mammalian cells of a gene linked to the poliovirus 5' noncoding region.
Translation in mammalian cells of a gene linked to the poliovirus 5' noncoding region.
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DOI：
10.1126/science.2839901
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发表时间：
1988-07
期刊：
影响因子：
56.9
通讯作者：
D. Trono;J. Pelletier;N. Sonenberg;D. Baltimore
中科院分区：
文献类型：
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作者：
D. Trono;J. Pelletier;N. Sonenberg;D. Baltimore
脊髓灰质炎病毒5'端742个核苷酸非编码区的中心部分（P区）允许RNA在缺少通常的5' 7-甲基鸟苷加帽基团的情况下启动蛋白质合成。将脊髓灰质炎病毒5'非编码区与报告基因融合并转染细胞。脊髓灰质炎病毒介导的帽依赖性蛋白质合成的抑制广泛增强了该基因的表达。通过体外实验验证了该构建体以帽非依赖性方式启动。整个P区的小病变阻断了其起始功能，这意味着一个连贯的功能单位，数百个核苷酸长，负责脊髓灰质炎病毒RNA的帽非依赖性起始。
The central portion (region P) of the 742-nucleotide noncoding 5' end of poliovirus allows the RNA to initiate protein synthesis in the absence of the usual 5' 7-methylguanosine capping group. Poliovirus 5' noncoding region was fused to a reporter gene and transfected into cells. There was extensive augmentation of the expression of this gene by poliovirus-mediated inhibition of cap-dependent protein synthesis. That the construct initiated in a cap-independent manner was verified through in vitro experiments. Small lesions throughout region P blocked its initiation function, implying that a coherent functional unit, hundreds of nucleotides long, is responsible for cap-independent initiation by poliovirus RNA.