The effect of aging on the seminiferous epithelium of the brown Norway rat.
The effect of aging on the seminiferous epithelium of the brown Norway rat.
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DOI：
10.1002/j.1939-4640.1993.tb01661.x
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发表时间：
1993-03
影响因子：
--
通讯作者：
William W. Wright;Cara Fiore;B. Zirkin
中科院分区：
文献类型：
--
作者：
William W. Wright;Cara Fiore;B. Zirkin
哺乳动物睾丸的老化通常伴随着生殖细胞的损失，结果是每日精子产量减少。目前尚不清楚细胞损失是否是生殖细胞中与衰老相关的变化的结果，或者在通常支持生殖发育和分化的支持细胞中是否也存在与衰老相关的变化。为了开始比较年龄对生殖细胞和支持细胞的影响，我们检查了6、12、18、21和24月龄的棕色挪威大鼠的生精小管退化频率和三种支持细胞产物（SGP-2、转铁蛋白和环蛋白-2（CP-2）/组织蛋白酶L）转录物的睾丸总含量。组织学分析显示，生精上皮从6至12个月的年龄没有变化。然而，从12月龄到24月龄，正常小管的百分比从95%逐渐下降到15%，而完全退化的小管（每个小管横截面不含生殖细胞）的百分比从0%增加到78%。在我们对特定支持细胞转录物的分析中，我们注意到从6个月到24个月，SGP-2 mRNA的总睾丸含量没有变化。然而，总睾丸转铁蛋白mRNA的含量从6至18个月不变，但增加了24个月的6个月大的睾丸内容的368%。相比之下，总睾丸CP-2/组织蛋白酶L mRNA的含量从6至12个月不变，但减少了24个月的6个月大的睾丸内容的58%。（250字处删节）
Aging of the mammalian testis is often accompanied by loss of germ cells and, as a result, decreased daily sperm production. It is currently unknown whether cell loss is the result of aging-related changes in germ cells or whether there are also aging-related changes in the Sertoli cells that normally support germ development and differentiation. To begin to compare the effects of age on germ cells and on Sertoli cells, we examined brown Norway rats of 6, 12, 18, 21, and 24 months of age for the frequency of seminiferous tubule regression and total testis contents of transcripts for three Sertoli cell products: SGP-2, transferrin, and cyclic protein-2 (CP-2)/cathepsin L. Histological analysis revealed no changes in the seminiferous epithelium from 6 to 12 months of age. However, from 12 to 24 months of age, the percentage of normal tubules gradually decreased from 95% to 15% of the total while the percentage of fully regressed tubules (containing no germ cells per tubule cross section) increased from 0% to 78%. In our analysis of specific Sertoli cell transcripts, we noted no change in total testis content of SGP-2 mRNA from 6 to 24 months. However, total testis content of transferrin mRNA was unchanged from 6 to 18 months, but increased by 24 months to 368% of the content of a 6-month-old testis. In contrast, total testis content of CP-2/cathepsin L mRNA was unchanged from 6 to 12 months, but decreased by 24 months to 58% of the content of a 6-month-old testis.(ABSTRACT TRUNCATED AT 250 WORDS)