Developing a Microloading Platform for Applications in Mechanotransduction Research
Developing a Microloading Platform for Applications in Mechanotransduction Research
复制标题
开发用于机械传导研究应用的微加载平台
DOI：
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
M. Saunders
中科院分区：
文献类型：
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作者：
Karan Shah;S. L. York;P. Sethu;M. Saunders
骨细胞机械转导涉及骨细胞感知和协调其活动以响应机械负荷的过程。经典的机械传递模型包括平行板流动和锥形粘度计系统。这些系统将液体流动应用于骨细胞，以研究液体剪切对细胞活动的影响。在这项研究中，开发了一种利用可变形膜使骨细胞处于复杂变形状态的微驱动加载装置。为了模拟加载分布，利用有限元分析，通过力学测试确定了聚二甲基硅氧烷(PDMS)的材料特性，以模拟细胞衬底和加载平台，以施加准确、可重复性和生理上合适的加载。
Bone cell mechanotransduction involves the process by which bone cells sense and coordinate their activity in response to mechanical loading. Classic mechanotransduction models include the parallel plate flow and cone viscometer systems. These systems apply fluid flow to bone cells to study the effects of fluid shear on cellular activity. In this study a microactuated loading device to subject bone cells to a complex state of deformation utilizing a deformable membrane was developed. To model the loading profile, finite element analysis was utilized with material properties of polydimethylsiloxane (PDMS) determined from mechanical testing to model a cell substrate and loading platen to apply accurate, reproducible and physiologically appropriate loading.