2 TYPES OF HUMAN MAST-CELLS THAT HAVE DISTINCT NEUTRAL PROTEASE COMPOSITIONS
2 TYPES OF HUMAN MAST-CELLS THAT HAVE DISTINCT NEUTRAL PROTEASE COMPOSITIONS
复制标题
DOI：
10.1073/pnas.83.12.4464
复制
发表时间：
1986-06-01
影响因子：
11.1
通讯作者：
SCHWARTZ, LB
中科院分区：
文献类型：
--
作者：
IRANI, AA;SCHECHTER, NM;SCHWARTZ, LB
通过免疫组织化学技术在皮肤、肺和小肠中鉴定出两种人类肥大细胞类型。一类含有中性蛋白酶、类胰蛋白酶和胰凝乳蛋白酶，被称为TC肥大细胞。第二种类型仅含有类胰蛋白酶，称为肥大 T 细胞。卡诺氏液比福尔马林对这两种类型的固定效果更好。皮肤中T型肥大细胞占12%；小肠粘膜98个，粘膜下层13个；支气管/细支气管上皮下约 77 个，支气管/细支气管上皮约 97 个，肺泡中约 93 个。分散的肺细胞含有 90% 的肥大 T 细胞。 TC肥大细胞的平均面积(76μm2)略大于T肥大细胞的平均面积(66μm2)；然而，存在如此广泛的重叠，以至于无法根据大小区分属于不同类型的单个肥大细胞。具有不同蛋白酶组成的人类肥大细胞类型的识别表明人类肥大细胞介导的反应比迄今为止所认识到的更高水平的复杂性。
Two human mast cell types were identified by immunohistochemical techniques in skin, lung, and small intestine. One type contains the neutral proteases, tryptase and chymotryptic proteinase, and is termed the TC mast cell. The second type contains only tryptase and is termed the T mast cell. Both types are fixed better by Carnoy''s fluid than by formalin. The percentage of mast cells accounted for by the T type was 12 in skin; 98 in mucosa and 13 in submucosa of small intestine; and 77 in bronchial/bronchiolar subepithelium, about 97 in bronchial/bronchiolar epithelium, and 93 in alveoli of lung. Dispersed lung cells contained 90% T mast cells. The mean area of TC mast cells (76 .mu.m2) was slightly larger than that of T mast cells (66 .mu.m2); however, there was such extensive overlap that individual mast cells belonging to different types could not be distinguished on the basis of size. The recognition of human mast cell types with distinct protease compositions suggests a higher level of complexity of human mast cell-mediated reactions than heretofore appreciated.