Generation of kinins in synovial fluid from patients with arthropathy
Generation of kinins in synovial fluid from patients with arthropathy
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DOI：
10.1016/s0162-3109(97)00023-4
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发表时间：
1997-06-01
期刊：
影响因子：
--
通讯作者：
Bhoola, KD
中科院分区：
文献类型：
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作者：
Bond, AP;Lemon, M;Bhoola, KD
描述了一种酶联免疫吸附测定方法，用于测量类风湿和骨关节炎（RA和GA）患者滑液中激肽的形成。在存在激肽形成（激肽原酶）和激肽代谢（激肽酶）抑制剂的情况下，收集的滑液中基础激肽浓度小于6 ng/ml。在单独存在激肽酶抑制剂的滑液孵育期间，激肽在前10分钟内迅速产生，但由于内源性激肽酶的抑制，在30分钟内完全停止产生；快速期早期激肽的生成速率与高原激肽浓度有良好的相关性。15例OA和RA患者滑液中平台激肽水平从98到427 ng/ml不等，中位值为148 ng/ml。本研究清楚地表明，来自关节炎患者的滑液具有产生激肽的能力。虽然患者数量很少，但体外产生的激肽量变化很大，并且在更大的患者群体中，关节内激肽形成与临床特征之间的关系可能变得明显。该技术也可用于研究其他生物系统，其中已提出激肽的作用。
An enzyme-linked immunosorbent assay method is described for the measurement of kinin formation in synovial fluid from patients with rheumatoid and osteoarthritis (RA and GA). Basal kinin concentrations were less than 6 ng/ml in synovial fluid collected in the presence of inhibitors of kinin forming (kininogenase) and kinin metabolising (kininase) enzymes. During incubation of synovial fluid in the presence of kininase inhibitors alone, kinins were produced rapidly over the first 10 min, but production ceased completely within 30 min due to inhibition of the endogenous kininogenases; the rate of kinin generation during the early rapid phase correlated well with the plateau kinin concentration. Plateau kinin levels in synovial fluid from 15 patients with OA and RA ranged from 98 to 427 ng/ml, with a median value of 148 ng/ml. This study demonstrates clearly that synovial fluid from arthritis patients has the capacity to produce kinins. Although the number of patients was small, the amount of kinin generated in vitro varied over a wide range and a relationship between intra-articular kinin formation and clinical features may become apparent in a larger group of patients, The technique could also be used to investigate other biological systems in which a role has been proposed for kinins.