Imaging regions of transport across human stratum corneum during high-voltage and low-voltage exposures.
Imaging regions of transport across human stratum corneum during high-voltage and low-voltage exposures.
复制标题
在高电压和低电压暴露期间对穿过人体角质层的运输区域进行成像。
DOI：
10.1021/js960020s
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Cullander,C
中科院分区：
文献类型：
--
作者：
Prausnitz,MR;Gimm,JA;Guy,RH;Langer,R;Weaver,JC;Cullander,C
扫描共聚焦荧光显微镜被用来成像在持续的低电压(离子导入)和脉冲高电压暴露期间钙黄绿素通过人的角质层运输的局部区域。按照电学方案，成像显示了荧光区域，这些区域被解释为荧光探针(钙黄绿素)进入角质层的位置。钙黄绿素的电辅助转运，无论是通过电泳法还是高压脉冲，似乎都是通过细胞间的途径发生的，在某种程度上，跨细胞途径进入角质层的局部区域，这些区域与附件无关。与使用的最高电压脉冲(整个皮肤300伏)相关的独特之处是出现了包含非荧光内部的明亮的小荧光区域，即荧光“环”。我们提出的证据表明，黑暗的内部代表了脉冲期间发生运输的部位，但在成像时钙黄绿素不再存在。还观察到带电微球进入角质层的情况。
Scanning confocal fluorescence microscopy was used to image localized regions of calcein transport across human stratum corneum during constant low-voltage (iontophoresis) and pulsed high-voltage exposures. Following an electrical protocol, imaging revealed regions of fluorescence which were interpreted as sites where transport of a fluorescent probe (calcein) into the stratum corneum had taken place. Electrically-assisted transport of calcein, whether enhanced by ionto-phoresis or high-voltage pulsing, appears to occur through intercellular and, to some extent, transcellular pathways into localized regions of stratum corneum that are not associated with appendages. Uniquely associated with the highest voltage pulses used (300 V across the skin) was the appearance of small, brightly fluorescent areas containing nonfluorescent interiors, i.e., fluorescent “rings”. We present evidence which suggests that the dark interiors represent sites through which transport occurred during pulsing, but where calcein was no longer present at the time of imaging. Transport of charged microspheres into the stratum corneum was also observed.