DIFFERENTIAL CORTICOSTEROID REGULATION OF TYPE-II GLUCOCORTICOID RECEPTOR-LIKE IMMUNOREACTIVITY IN THE RAT CENTRAL-NERVOUS-SYSTEM - TOPOGRAPHY AND IMPLICATIONS
DIFFERENTIAL CORTICOSTEROID REGULATION OF TYPE-II GLUCOCORTICOID RECEPTOR-LIKE IMMUNOREACTIVITY IN THE RAT CENTRAL-NERVOUS-SYSTEM - TOPOGRAPHY AND IMPLICATIONS
复制标题
DOI：
10.1210/endo-129-1-226
复制
发表时间：
1991-07-01
期刊：
影响因子：
4.8
通讯作者：
HARLAN, RE
中科院分区：
文献类型：
--
作者：
AHIMA, RS;HARLAN, RE
中枢神经系统神经元II型糖皮质激素受体样免疫反应在强度和相对密度上表现出异质性。这可能预测神经细胞对皮质类固醇免疫反应的区域反应的变化。我们研究了肾上腺切除术和皮质类固醇治疗后大鼠中枢神经系统免疫反应性细胞内位置的变化，并对海马和尾状壳核神经元核免疫反应性的变化进行了详细的统计分析。观察到三种类型的反应。大部份神经元，分类为A型，在完整大鼠中表现出主要的核免疫反应性，在肾上腺切除术后失去核免疫反应性并最终失去胞质免疫反应性，分别在皮质酮治疗5分钟和醛固酮治疗2小时内恢复核免疫反应性。完整大鼠海马、纹状体、隔和缰中有一类B型神经元，在肾上腺切除术后表现出强烈的细胞质免疫反应，在皮质酮治疗后和醛固酮治疗后迅速消失。小脑皮层浦肯野神经元亚群，分类为C型，在肾上腺切除术后表现出强烈的细胞质免疫反应性，对皮质酮的反应增加，对醛固酮无反应。
Neuronal type II glucocorticoid receptor-like immunoreactivity in the central nervous system shows heterogeneity in intensities and relative densities. This might predict variations in the regional responses of neuronal immunoreactivity to corticosteroids. We investigated changes in the intracellular location of immunoreactivity in the rat central nervous system after adrenalectomy and corticosteroid treatment, and carried out detailed statistical analysis of changes in neuronal nuclear immunoreactivity in the hippocampus and caudate-putamen. Three types of responses were observed. The majority of neurons, classified type A, showed a predominant nuclear immunoreactivity in intact rats, lost nuclear and eventually cytoplasmic immunoreactivity after adrenalectomy, and regained nuclear immunoreactivity within 5 min of corticosterone and 2 h of aldosterone treatment, respectively. A subgroup of neurons in the hippocampus, striatum, septum, and habenula, classified type B, were not immunoreactive in intact rats, showed intense cytoplasmic immunoreactivity after adrenalectomy, and disappeared rapidly after corticosterone treatment and later in response to aldosterone. A subgroup of vermal cerebellar Purkinje neurons, classified type C, developed an intense cytoplasmic immunoreactivity after adrenalectomy, increased in number in response to corticosterone, and did not respond to aldosterone.