Immunohistological study for estrogenic activities of nitrophenols in diesel exhaust particles
Immunohistological study for estrogenic activities of nitrophenols in diesel exhaust particles
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DOI：
10.1385/endo:27:1:033
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发表时间：
2005-06-01
期刊：
影响因子：
3.7
通讯作者：
Taya, K
中科院分区：
文献类型：
--
作者：
Furuta, C;Li, CM;Taya, K
我们最近从柴油机尾气颗粒（DEP）中分离出3-甲基-4-硝基苯酚（4-硝基-间甲酚；PNMC）和4-硝基-3-苯基苯酚（PNMPP），并将它们鉴定为血管扩张剂和异雌激素。采用免疫组织化学染色法对去势25日龄未成熟雌性大鼠皮下注射PNMC和PNMPP 2 d子宫腔上皮中的增殖细胞核抗原(PCNA)进一步检测PNMC和PNMPP的雌激素活性。与对照组相比，接受 10 和 100 mg/kg PNMC 和 0.1 mg/kg PNMPP 的动物子宫腔上皮的 PCNA 阳性细胞显着增加。这些结果清楚地显示了 PNMC 和 PNMPP 通过子宫腔上皮细胞增殖产生的雌激素活性。
We recently isolated 3-methyl-4-nitrophenol (4-nitro-m-cresol; PNMC) and 4-nitro-3-phenyl phenol (PNMPP) from diesel exhaust particles (DEP) and identified them as vasodilators and xenoestrogens. The estrogenic activity of PNMC and PNMPP was further examined by using immunohistochemical staining of proliferating cell nuclear antigen (PCNA) in uterine luminal epithelium of ovariectomized 25-d-old immature female rats injected with PNMC and PNMPP subcutaneously for 2 d. Significant increases were observed in uterine luminal epithelium in PCNA positive cells of animals receiving 10 and 100 mg/kg PNMC and 0.1 mg/kg PNMPP compared with controls. These results clearly show the estrogenic activity of PNMC and PNMPP by cell proliferation on the uterine luminal epithelium.