The CesA gene family of barley. Quantitative analysis of transcripts reveals two groups of co-expressed genes
The CesA gene family of barley. Quantitative analysis of transcripts reveals two groups of co-expressed genes
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DOI：
10.1104/pp.103.032904
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发表时间：
2004-01-01
期刊：
影响因子：
7.4
通讯作者：
Fincher, GB
中科院分区：
文献类型：
--
作者：
Burton, RA;Shirley, NJ;Fincher, GB
来自cDNA和基因组克隆的序列数据，加上表达序列标签数据库的分析，表明来自大麦（Hordeum vulgare）的CesA（纤维素合酶）基因家族至少有8个成员，它们分布在不同的区域，基因组定量聚合酶链反应已被用来确定在营养和花组织中，在不同的发展阶段，单个HvCesA基因的mRNA转录的相对丰度。为了确保准确的表达谱分析，已将多个内部对照基因转录物的几何平均值应用于转录物丰度的标准化。总HvCesA mRNA水平最高的胚芽鞘，根，茎和低得多的花组织，早期发育的粮食，并在伸长区的叶。在大多数组织中，HvCesA 1、HvCesA 2和HvCesA 6占主导地位，它们的相对丰度非常相似;这些基因似乎是协同转录的。第二组，包括HvCesA 4，HvCesA 7，和HvCesA 8，也似乎是协调转录，最明显的是在成熟的茎和根组织。HvCesA 3表达模式不属于这两组中的任一组，并且HvCesA 5转录物水平在所有组织中都极低。因此，就mRNA丰度而言，HvCesA基因分为两个一般的三个基因组，并且该组的共表达将它们的产物鉴定为参与质膜上纤维素生物合成的植物的候选物。系统发育分析允许这两组基因与参与初生壁和次生壁合成的拟南芥CesA基因连锁。
Sequence data from cDNA and genomic clones, coupled with analyses of expressed sequence tag databases, indicate that the CesA (cellulose synthase) gene family from barley (Hordeum vulgare) has at least eight members, which are distributed across., the genome. Quantitative polymerase chain reaction has been used to determine the relative abundance of mRNA transcripts for individual HvCesA genes in vegetative and floral tissues, at different stages of development. To ensure accurate expression profiling, geometric averaging of multiple internal control gene transcripts has been applied for the normalization of transcript abundance. Total HvCesA mRNA levels are highest in coleoptiles, roots, and stems and much lower in floral tissues, early developing grain, and in the elongation zone of leaves. In most tissues, HvCesA1, HvCesA2, and HvCesA6 predominate, and their relative abundance is very similar; these genes appear to be coordinately transcribed. A second group, comprising HvCesA4, HvCesA7, and HvCesA8, also appears to be coordinately transcribed, most obviously in maturing stem and root tissues. The HvCesA3 expression pattern does not fall into either of these two groups, and HvCesA5 transcript levels are extremely low in all tissues. Thus, the HvCesA genes fall into two general groups of three genes with respect to mRNA abundance, and the co-expression of the groups identifies their Products as candidates for the rosettes that are involved in cellulose biosynthesis at the plasma membrane. Phylogenetic analysis allows the two groups of genes to be linked with orthologous Arabidopsis CesA genes that have been implicated in primary and secondary wall synthesis.