Expression of c-met proto-oncogene in primary colorectal cancer and liver metastases
Expression of c-met proto-oncogene in primary colorectal cancer and liver metastases
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DOI：
10.1093/jjco/27.6.378
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发表时间：
1997-12-01
影响因子：
2.4
通讯作者：
Sugano, K
中科院分区：
文献类型：
--
作者：
Fujita, S;Sugano, K
我们使用逆转录酶-聚合酶链式反应方法检测了 27 例结直肠癌和 10 例肝转移瘤组织中 c-met mRNA 的表达。对这些组织中c-met mRNA的表达量进行定量，并计算c-met mRNA相对于10（8.0）份β-actin mRNA的拷贝数，癌组织和正常粘膜中c-met mRNA的平均拷贝数分别为10（5.5）和10（4.5），癌组织中c-met的表达显着高于正常粘膜（P < 0.0001），在22个样本中的20个中哪个c-met表达 对肿瘤组织和相应正常组织进行检测，癌组织中c-met过表达，c-met表达与临床病理背景无相关性，10例肝转移组织和正常肝组织中c-met mRNA的平均拷贝数分别为10(6.1)和10(6.2)。尽管转移组织中c-met的表达高于原发癌组织，但增加幅度并不具有统计学意义。 In three of four patients with synchronous liver metastases, c-met was overexpressed in the metastatic tissue compared with that in the corresponding primary cancer tissue, These results show that c-met is overexpressed in both primary colorectal cancer and liver metastases and suggest that c-met plays a role in the development of colorectal cancer liver metastases.
We have examined the expression of c-met mRNA in tissue from 27 colorectal cancers and ten liver metastases using the reverse transcriptase-polymerase chain reaction method. The expression of c-met mRNA in these tissues was quantified and the copy number of c-met mRNA to 10(8.0) copies of beta-actin mRNA was calculated, Mean copy numbers of c-met mRNA in cancer tissue and normal mucosa were 10(5.5) and 10(4.5) respectively, The c-met expression of cancer was significantly higher than that of normal mucosa (P < 0.0001), In 20 of 22 samples in which c-met expression of both tumor and corresponding normal tissue were examined, c-met was overexpressed in the cancer tissue, No correlation was found between c-met expression and the clinicopathologic background, The mean copy numbers of c-met mRNA in the tissue from the ten liver metastases and normal liver were 10(6.1) and 10(6.2) respectively. Although c-met expression in metastatic tissue was higher than that in the primary cancer tissue, the increase was not statistically significant. In three of four patients with synchronous liver metastases, c-met was overexpressed in the metastatic tissue compared with that in the corresponding primary cancer tissue, These results show that c-met is overexpressed in both primary colorectal cancer and liver metastases and suggest that c-met plays a role in the development of colorectal cancer liver metastases.