Detection and measurement of the alkaloid peramine in endophyte-infected grasses
Detection and measurement of the alkaloid peramine in endophyte-infected grasses
复制标题
内生菌感染草中生物碱波胺的检测和测定
DOI：
10.1016/s0021-9673(01)84460-5
复制
发表时间：
1989
影响因子：
4.1
通讯作者：
G. Latch
中科院分区：
文献类型：
--
作者：
B. Tapper;D. Rowan;G. Latch
描述了两种检测和测量波胺的方法，波胺是一种来自内生菌感染草的生物碱，具有昆虫摄食威慑活性。两种程序均涉及对提取物进行离子交换处理，然后进行薄层色谱法并通过艾利希试剂进行检测，或者通过反相高效液相色谱法进行定量并在 280 nm 处进行 UV 检测。
Two methods are described for the detection and measurement of peramine, an alkaloid from endophyte-infected grasses with insect feeding deterrent activity. Both procedures involve ion-exchange work up of extracts followed by either thin-layer chromatography with detection by Ehrlich's reagent or quantitation by reversed-phase high-performance liquid chromatography with UV detection at 280 nm.