Effects of Interleukin-31 on MUC5AC Gene Expression in Nasal Allergic Inflammation
Effects of Interleukin-31 on MUC5AC Gene Expression in Nasal Allergic Inflammation
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DOI：
10.1159/000346609
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发表时间：
2013-01-01
期刊：
影响因子：
3.1
通讯作者：
Takeuchi, Kazuhiko
中科院分区：
文献类型：
--
作者：
Shah, Said Ahmad;Ishinaga, Hajime;Takeuchi, Kazuhiko
背景：白细胞介素-31 （IL-31）是一种新发现的T辅助淋巴细胞来源的细胞因子，在变应性炎症中起重要作用。然而，IL-31对鼻腔变应性炎症中粘液生成的影响尚不清楚。目的：探讨IL-31对变应性鼻炎患者及人气道上皮细胞黏液蛋白基因（MUC5AC）表达的影响。方法：应用免疫组织化学方法检测变应性鼻炎和非变应性鼻炎患者下鼻甲组织中IL-31和IL-31受体A （IL-31 RA）的表达水平。采用MUC5AC荧光素酶报告基因法测定人上皮细胞HM3-MUC5AC中il -31诱导的MUC5AC基因表达，并采用实时定量聚合酶链反应法测定人气道上皮细胞A549中MUC5AC基因表达。结果：IL-31RA主要定位于粘膜下腺体，在变应性鼻炎中表达上调。粘膜下组织检测到IL-31，变应性炎症中IL-31升高。IL-31刺激可诱导HM3-MUC5AC和A549细胞MUC5AC基因表达。IL-31与Th2细胞因子共同影响HM3-MUC5AC细胞MUC5AC基因的表达。结论：IL-31和IL-31 RA在变应性鼻炎患者中表达上调，并诱导人气道上皮细胞MUC5AC基因表达。这些发现提示IL-31在鼻腔变应性炎症中粘液过量产生中起重要作用。巴塞尔S. Karger股份有限公司版权所有
Background: Interleukin-31 (IL-31) is a newly discovered T helper lymphocyte-derived cytokine that plays an important role in allergic inflammation. However, the effects of IL-31 on mucus production in nasal allergic inflammation are completely unknown. Objective: To investigate the effects of IL-31 on mucin gene expression (MUC5AC) in patients with allergic rhinitis and in human airway epithelial cells. Methods: Expression levels of IL-31 and IL-31 receptor A (IL-31 RA) were evaluated in the inferior turbinate of patients with allergic rhinitis and non-allergic rhinitis with innmunohistochemistry. IL-31-induced MUC5AC gene expression was measured with a MUC5AC luciferase reporter assay in human epithelial HM3-MUC5AC cells and quantified by quantitative real-time polymerase chain reaction in human airway epithelial A549 cells. Results: IL-31RA was primarily localized in submucosal glands and upregulated in allergic rhinitis. IL-31 was detected in submucosal tissue and increased in allergic inflammation. MUC5AC gene expression was induced by IL-31 stimulation both in HM3-MUC5AC and A549 cells. Additionally, IL-31 cooperated with Th2 cytokines on MUC5AC gene expression in HM3-MUC5AC cells. Conclusion: IL-31 and IL-31 RA are upregulated in patients with allergic rhinitis, and induce MUC5AC gene expression in human airway epithelial cells. These findings suggest that IL-31 plays an important role in mucus overproduction in nasal allergic inflammation. Copyright (C) 2013 S. Karger AG, Basel