Time course of capillary regression and an expression balance between vascular endothelial growth factor-A and thrombospondin-1 in the soleus muscle of hindlimb unloaded rats
Time course of capillary regression and an expression balance between vascular endothelial growth factor-A and thrombospondin-1 in the soleus muscle of hindlimb unloaded rats
复制标题
后肢无负荷大鼠比目鱼肌毛细血管消退的时程及血管内皮生长因子-A和血小板反应蛋白-1的表达平衡
DOI：
10.1002/mus.27478
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发表时间：
2022
期刊：
影响因子：
3.4
通讯作者：
Fujino H.
中科院分区：
文献类型：
--
作者：
Tanaka M;Kanazashi M;Kondo H;Fujino H.
前言/目的骨骼肌毛细血管随停用而退化；然而，有关废弃肌肉中促血管生成因子和抗血管生成因子表达随时间变化的信息有限。本研究旨在阐明后肢去负重大鼠比目鱼肌毛细血管形成、促血管生成血管内皮生长因子-A(VEGF-A)和抗血管新生血栓反应蛋白-1(TSP-1)的时间依赖性变化。方法8周龄雄性SD大鼠随机分为4组，分别在0、1、2、3周时不同的后肢去负重时间(HU)。术后1周血管内皮生长因子-A蛋白表达水平低于术后0周。TSP-1蛋白表达水平在1周高于0周，但在2周和3周差异无统计学意义。此外，用二氢乙锭荧光强度评价的活性氧含量在2周和3周高于0周。在HU早期，TSP-1可能在毛细血管回归中起重要作用。然而，当HU延长较长时间时，血管内皮生长因子-A降低和/或氧化应激增加可能更多地参与毛细血管退缩。
Introduction/AimsSkeletal muscle capillaries regress with disuse; however, information on time‐dependent changes in the expression of pro‐ and anti‐angiogenic factors in disused muscle is limited. This study aimed to clarify time‐dependent changes in skeletal muscle capillarization, pro‐angiogenic vascular endothelial growth factor‐A (VEGF‐A), and anti‐angiogenic thrombospondin‐1 (TSP‐1) in the soleus muscle of hindlimb unloaded rat.MethodsEight‐week‐old male Sprague Dawley rats were randomly divided into four groups corresponding to different hindlimb unloading (HU) duration at 0, 1, 2, and 3 wk.ResultsMuscle atrophy and capillary regression worsened in the soleus muscle with longer periods of HU. The VEGF‐A protein expression level was lower at week 1 than at week 0. In addition, the value at week 3 was also lower than those at weeks 0, 1, and 2. The TSP‐1 protein expression level was higher at week 1 than that at week 0 but was similar at weeks 2 and 3. Moreover, reactive oxygen species, assessed by dihydroethidium fluorescence intensity on cryosection, were higher at weeks 2 and 3 than that at week 0.DiscussionDepending on the HU period, VEGF‐A and TSP‐1 showed different expression patterns. In the early HU phase, TSP‐1 may play an important role in capillary regression. However, when HU extends for a longer period, decreased VEGF‐A, and/or increased oxidative stress may be more involved in capillary regression.