Distinct genotype and antigenicity among genogroup II sapoviruses
Distinct genotype and antigenicity among genogroup II sapoviruses
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DOI：
10.1111/j.1348-0421.2009.00133.x
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发表时间：
2009-07-01
影响因子：
2.6
通讯作者：
Takeda, Naokazu
中科院分区：
文献类型：
--
作者：
Oka, Tomoichiro;Miyashita, Kana;Takeda, Naokazu
SaV是一种急性胃肠炎病原体，分为5个基因群，从GI到GV。然而，SaV抗原性与遗传簇之间的关系尚不完全清楚。我们最近鉴定了两种GII SaV菌株Mc10和C12，它们根据聚合酶被归为同一簇，但根据衣壳被归为不同的簇。为了评估这两种菌株的抗原性差异，我们在哺乳动物细胞中表达了VLP。抗原ELISA首次证明了同一GII SaV基因组的菌株，但在不同的聚集中，具有不同的抗原性。
SaV, a pathogen of acute gastroenteritis, is divided into five genogroups, GI to GV. However, the relation between SaV antigenicity and genetic clusters is not fully understood. We have recently identified two GII SaV strains, Mc10 and C12, which are grouped into the same cluster based on the polymerase but are grouped into distinct clusters based on the capsid. To evaluate the difference in antigenicity between these two strains, VLP were expressed in mammalian cells. An antigen ELISA demonstrated for the first time that strains in the same GII SaV genogroup, but within different clusters, have distinct antigenicities.