Easy-to-use equipment for the accurate microinjection of nanoliter volumes into the nuclei of amphibian oocytes.
Easy-to-use equipment for the accurate microinjection of nanoliter volumes into the nuclei of amphibian oocytes.
复制标题
易于使用的设备，可将纳升体积精确显微注射到两栖动物卵母细胞的细胞核中。
DOI：
10.1016/0003-2697(81)90485-1
复制
发表时间：
1981
影响因子：
2.9
通讯作者：
Mertz,JE
中科院分区：
文献类型：
--
作者：
Stephens,DL;Miller,TJ;Silver,L;Zipser,D;Mertz,JE
Microinjection of Xenopus oocytes has become a valuable technique for studying many aspects of control of eucaryotic gene expression. Previously published methods for controlling and monitoring the volume of sample injected into oocytes are time consuming and tedious and require considerable expertise to obtain reproducible results. The construction and manner of usage of micropipets are the most critical parts of a successful injection technique. Methods for the construction of micropipets and ways to assess the accuracy and precision of the injection of nanoliter volumes into amphibian oocytes are described. Construction of pipets is done almost entirely using instruments that are relatively inexpensive and allow for a high degree of reproducibility. Micropipets made in this manner have several advantages: (1) They inflict little damage on the oocytes during the injection process; greater than 95% of the oocytes survive after microinjection. (2) Oocytes do not require defolliculation prior to microinjection. (3) One micropipet can be used to inject as many as 100 oocytes before needing to be refilled with sample and without acquiring a blockage. Using the microinjection techniques described, the accuracy of sample delivery was within 9% of the mean and the precision increased with increasing volume: the standard deviations of volumes in the 5-nl range were within 9% of the mean and volumes in the range 10–50 nl were within 4–6% of the mean.
登录
查看更多内容
DOI：
--
发表时间：
1960
期刊：
Journal of embryology and experimental morphology
影响因子：
--
作者：
T. Elsdale;J. Gurdon;M. Fischberg
通讯作者：
M. Fischberg
注射到非洲爪蟾卵母细胞中的 5S DNA 转录。
影响因子：
2.7
作者：
J. Gurdon;D. D. Brown
通讯作者：
D. D. Brown
非洲爪蟾卵母细胞中禽肉瘤病毒 RNA 的翻译。
DOI：
--
发表时间：
1979
期刊：
Biochemical and Biophysical Research Communications - BBRC
影响因子：
--
作者：
R. Katz;G. Maniatis;R. Guntaka
通讯作者：
R. Guntaka
显微注射青蛙卵母细胞的克隆酵母酪氨酸 tRNA 基因的转录和加工
影响因子：
64.8
作者：
E. D. Robertis;M. Olson
通讯作者：
M. Olson
爪蟾卵母细胞中鲤鱼胰岛素原的合成。
DOI：
10.1111/j.1432-1033.1978.tb12370.x
发表时间：
1978
期刊：
European journal of biochemistry
影响因子：
--
作者：
T. Rapoport;B. Thiele;S. Prehn;G. Marbaix;Y. Cleuter;E. Hubert;G. Huez
通讯作者：
G. Huez