Monocytes in cystic fibrosis: responsiveness to microbial stimuli.
Monocytes in cystic fibrosis: responsiveness to microbial stimuli.
复制标题
囊性纤维化中的单核细胞：对微生物刺激的反应。
DOI：
10.1002/ppul.1950080309
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发表时间：
1990
影响因子：
3.1
通讯作者：
Stern,RC
中科院分区：
文献类型：
--
作者：
Thomassen,MJ;Barna,BP;Farmer,MO;Doershuk,CF;Stern,RC
从19例囊性纤维化（CF）患者和年龄匹配的配对正常人中获得外周血单核细胞。用鼠伤寒沙门氏菌和铜绿假单胞菌脂多糖（LPS）刺激前后的超氧阴离子生成来测定这些细胞的氧化代谢反应。CF单核细胞自发产生超氧阴离子（14.1±2.1 SEM纳米摩尔超氧阴离子/ 106个单核细胞/180分钟，n = 12）略高于正常单核细胞（9.5±1.4,n = 12），P= 0.009。CF与正常人在LPS刺激下产生的超氧阴离子没有差异（CF = 33.5±4.6,n = 13；正常人= 33.8±4.2,n = 13）。此外，CF单核细胞对两种p均有反应。aeruginosaandS。鼠伤寒杆菌lps刺激以及重组干扰素- γ。在自体血清和正常血清中，CF单核细胞的超氧阴离子产生是相当的，并且p定殖患者的反应。aeruginosaandP。塞帕恰亚没有异议。我们得出结论，CF单核细胞代谢水平略有增加，尽管慢性感染，但能够进一步响应外源性微生物刺激。儿科肺科杂志1990；181年8:178所致。
Peripheral blood monocytes were obtained from 19 patients with cystic fibrosis (CF) and age‐matched paired normal individuals. The oxidative metabolic response of these cells was measured by superoxide anion production before and after stimulation withSalmonella typhimuriumorPseudomonas aeruginosalipopolysaccharide (LPS). CF monocytes showed slightly greater spontaneous superoxide anion production (14.1 ± 2.1 SEM nanomoles superoxide anion/ 106monocytes/180 min; n = 12) than normal monocytes (9.5 ± 1.4; n = 12),P= 0.009. No differences between CF and normals were found in LPS‐stimulated superoxide anion production (CF = 33.5 ± 4.6, n = 13; normal = 33.8 ± 4.2, n = 13). Furthermore, CF monocytes responded to bothP. aeruginosaandS. typhimuriumLPS stimulation as well as to recombinant interferon‐gamma. Superoxide anion production of CF monocytes was comparable in autologous serum and in normal serum, and responses of patients colonized withP. aeruginosaandP. cepaciadid not differ. We conclude that CF monocytes have a slightly increased metabolic level and, despite chronic infection, are capable of a further response to exogenous microbial stimuli. Pediatr Pulmonol 1990; 8:178‐181.