Genetic diversity comparison between Chinese and Japanese soybeans (Glycine max (L.) Merr.) revealed by nuclear SSRs
Genetic diversity comparison between Chinese and Japanese soybeans (Glycine max (L.) Merr.) revealed by nuclear SSRs
复制标题
通过核SSR揭示中国和日本大豆（Glycine max (L.) Merr.）的遗传多样性比较
DOI：
10.1007/s10722-009-9465-8
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发表时间：
2010-02-01
影响因子：
2
通讯作者：
Qiu, Lijuan
中科院分区：
文献类型：
--
作者：
Guan, Rongxia;Chang, Ruzhen;Qiu, Lijuan
大豆（Glycine max（L.）梅尔）在中国和日本都有悠久的种植历史。为探讨中国大豆与日本大豆的亲缘关系，利用46个SSR标记对205份代表7个不同生态类型的中国大豆和39份来自不同地区的日本大豆进行了遗传分析。共检测到745个等位基因，平均每个位点16.2个等位基因。等位基因频率在0.002 ~ 0.554之间，平均为0.06。UPGMA聚类分析将中国大豆和日本大豆分开，表明两国大豆形成了不同的基因库。日本大豆与中国7个不同生态类型大豆的等位基因比较，中国大豆有164-200个等位基因，日本大豆有64-112个等位基因。SSR多样性分析结果表明，中国品种的遗传多样性高于日本品种。对数据进行分析，以解决遗传结构和解释群体之间的进化关系。将日本大豆、北方中国大豆、南方中国大豆和一个以华中地区大豆为主要种质的混合群划分为三个不同的类群。 进一步分析了5个与农艺性状相关的SSR位点，发现中国大豆和日本大豆的优势等位基因存在差异。本研究为我国大豆育种进一步利用日本大豆提供了重要参考。
Soybean (Glycine max (L.) Merr.) has a long planting history in both China and Japan. In order to investigate the genetic relationship between Chinese and Japanese soybeans, 205 Chinese soybean accessions, that represent the seven different soybean ecotypes, and 39 Japanese soybean accessions from various regions were analyzed by using 46 SSR loci. In total 745 alleles were detected with an average allele number of 16.2 per locus. The allelic frequencies varied from 0.002 to 0.554 with an average of 0.06. Cluster analysis with UPGMA separated the Chinese accessions from Japanese accessions, suggesting that soybean in these two countries form different gene pools. When comparing the Japanese soybean with that from seven different Chinese soybean ecotypes, 164–200 alleles were specific to the Chinese accessions and 64–112 specific to the Japanese accessions. The comparison of SSRs diversity revealed that accessions from China exhibited more genetic diversity than those from Japan. The data were analyzed to resolve the genetic structure and to interpret the evolutionary relationships between groups. Three distinct groups were identified, corresponding to Japanese soybean, Northern China soybean, Southern China soybean and a mixed group in which most accessions were from central China. We further analyzed five agronomic trait-related SSR loci and found that the preponderant alleles are different in Chinese and Japanese soybean. Our study provides important insights into further utilization of Japanese soybean in Chinese soybean breeding.