Fungi use the SakA (HogA) pathway for phytochrome-dependent light signalling
Fungi use the SakA (HogA) pathway for phytochrome-dependent light signalling
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DOI：
10.1038/nmicrobiol.2016.19
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发表时间：
2016-05-01
影响因子：
28.3
通讯作者：
Fischer, Reinhard
中科院分区：
文献类型：
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作者：
Yu, Zhenzhong;Armant, Olivier;Fischer, Reinhard
真菌的应激感应依赖于由双组分磷酸化接力和随后的MAP激酶级联组成的信号级联来触发基因表达。除了渗透或氧化应激外，真菌还能感知许多其他环境因素，其中之一就是光（1,2）。光控制形态发生途径，也控制次生代谢物如青霉素的产生。在这里，我们发现光敏色素依赖的光信号通路涉及到应力传感和渗透传感信号通路。在“盲”突变体筛选中，MAP激酶SakA（也称为HogA）通过全基因组测序被鉴定出来。光敏色素FphA与含组氨酸的磷酸转移蛋白YpdA物理相互作用，引起MAP激酶SakA的光依赖性磷酸化及其进入细胞核的穿梭。在没有光敏色素的情况下，SakA仍然对渗透胁迫有反应，但对光没有反应。因此，SakA通路整合了几个应激因素，可以被认为是环境信号的枢纽。
Stress-sensing in fungi depends on a signalling cascade comprised of a two-component phosphorylation relay plus a subsequent MAP kinase cascade to trigger gene expression. Besides osmotic or oxidative stress, fungi sense many other environmental factors, one of which is light(1,2). Light controls morphogenetic pathways but also the production of secondary metabolites such as penicillin. Here we show that phytochrome-dependent light signalling in Aspergillus nidulans involves the stress-sensing and osmosensing signalling pathway. In a screening for 'blind' mutants, the MAP kinase SakA (also known as HogA) was identified by wholegenome sequencing. The phytochrome FphA physically interacted with the histidine-containing phosphotransfer protein YpdA and caused light-dependent phosphorylation of the MAP kinase SakA and its shuttling into nuclei. In the absence of phytochrome, SakA still responded to osmotic stress but not to light. The SakA pathway thus integrates several stress factors and can be considered to be a hub for environmental signals.