Suppression of LX ribonuclease in tomato results in a delay of leaf senescence and abscission
Suppression of LX ribonuclease in tomato results in a delay of leaf senescence and abscission
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DOI：
10.1104/pp.106.080135
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发表时间：
2006-10-01
期刊：
影响因子：
7.4
通讯作者：
Burd, Shaul
中科院分区：
文献类型：
--
作者：
Lers, Amnon;Sonego, Lilian;Burd, Shaul
尽管T2核糖核酸酶（RNase）存在于不同的生物体中，并且在其蛋白质序列上是保守的，但其生物学功能通常是未知的。番茄（Lycopersicon esculentum） LX是一种T2/ s样rna酶，其表达与磷酸盐饥饿、乙烯反应、衰老和程序性细胞死亡有关。本研究以LX蛋白水平降低的反义番茄植株为研究对象，研究了LX的功能。当叶片衰老和LX的反义抑制延缓衰老进程时，LX蛋白水平通常升高。此外，我们观察到缺乏lx的植株叶片脱落明显延迟。这与LX蛋白在番茄成熟脱落带组织中的特异性诱导有关。LX RNase基因调控和反义抑制的结果表明，LX在脱落和衰老中都具有重要的功能作用。
Although present in different organisms and conserved in their protein sequence, the biological functions of T2 ribonucleases (RNase) are generally unknown. Tomato (Lycopersicon esculentum) LX is a T2/S-like RNase and its expression is known to be associated with phosphate starvation, ethylene responses, and senescence and programmed cell death. In this study, LX function was investigated using antisense tomato plants in which the LX protein level was reduced. LX protein levels normally become elevated when leaves senesce and antisense inhibition of LX retarded the progression of senescence. Moreover, we observed a marked delay of leaf abscission in LX-deficient plants. This correlated with specific induction of LX protein in the tomato mature abscission zone tissue. LX RNase gene regulation and the consequences of antisense inhibition indicate that LX has an important functional role in both abscission and senescence.