Calcite-forming Bacillus licheniformis Thriving on Underwater Speleothems of a Hydrothermal Cave
Calcite-forming Bacillus licheniformis Thriving on Underwater Speleothems of a Hydrothermal Cave
复制标题
形成方解石的地衣芽孢杆菌在热液洞穴的水下洞穴中繁衍生息
DOI：
10.1080/01490451.2018.1476626
复制
发表时间：
2018
影响因子：
2.3
通讯作者：
P. Alifano
中科院分区：
文献类型：
--
作者：
S. M. Tredici;A. Buccolieri;L. Tanini;Matteo Calcagnile;D. Manno;P. Alifano
摘要意大利的蒙苏马诺泰尔梅的水下环境是一个暗示性的环境，有不同类型的洞穴沉积物，包括沿着墙壁和屋顶的“叶状”和“花椰菜”结核，以及地板上的圆锥形假石笋。非常高的钙和溶解的二氧化碳水平，以及大量的碳酸钙沉淀是这个洞穴环境的特征。然而，生命在叶状结核表面上蓬勃发展，每平方厘米约有105个菌落形成单位的可培养异养微生物。地衣芽孢杆菌似乎是用于筛选的低营养培养基上的普遍可培养微生物。基于16 S rRNA基因的聚合酶链反应-单链构象多态性分析表明，第VI组Bacillaceae物种在水下洞穴沉积物的细菌群落中有很好的代表性。X-射线衍射和拉曼光谱数据表明，B。地衣分离物在体外产生了丰富的方解石微晶，其特征还在于通过扫描电子显微镜结合能量色散X射线光谱。方解石微晶的生产进行了分析，在不同的媒体（克里斯滕森的尿素琼脂和B4碳酸钙沉淀培养基）和培养条件下，它被发现是增强在低氧条件下的B4培养基中的硝酸盐补充。B4和B4-硝酸盐培养基也刺激B产生抗生素。地衣分离物，其通过微生物测定进行分析。
Abstract The underwater environment of Grotta Giusti (Monsummano Terme, Italy) is a suggestive setting with different types of speleothems including “leafy” and “cauliflower” concretions along the walls and roof, and conical pseudo-stalagmites on the floor. Very high calcium and dissolved CO2 levels, and massive calcium carbonate precipitation characterize this cave environment. Yet, life thrives on the leafy concretion surfaces with loads of cultivable heterotrophic microorganisms around 105 colony-forming units per cm2. Bacillus licheniformis appeared to be the prevalent cultivable microorganism on a low-nutrient medium that was used for screening. 16S rRNA gene-based polymerase chain reaction–single strand conformation polymorphism profiling indicated that Group VI Bacillaceae species was well represented in the bacterial community of underwater speleothems. Interpretation of X-ray diffraction spectra and Raman spectroscopy data indicated that the B. licheniformis isolate produced in vitro abundant calcite microcrystals that were also characterized by scanning electron microscopy coupled with energy dispersive X-ray spectroscopy. Production of calcite microcrystals was analyzed in different media (Christensen’s urea agar and B4 calcium carbonate precipitation medium) and incubation conditions, and it was found to be enhanced by nitrate supplement in B4 medium under low-oxygen conditions. B4 and B4-nitrate media also stimulated antibiotic production by the B. licheniformis isolate, which was analyzed by microbiological assays.
DOI：
10.1073/pnas.82.20.6955
发表时间：
1985-01-01
影响因子：
11.1
作者：
LANE, DJ;PACE, B;PACE, NR
通讯作者：
PACE, NR
影响因子：
5.6
作者：
Paulsen, IT;Nguyen, L;Saier, MH
通讯作者：
Saier, MH