Disruption of an Arabidopsis cytoplasmic ribosomal protein S13-homologous gene by transposon-mediated mutagenesis causes aberrant growth and development
Disruption of an Arabidopsis cytoplasmic ribosomal protein S13-homologous gene by transposon-mediated mutagenesis causes aberrant growth and development
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DOI：
10.1046/j.1365-313x.2000.00728.x
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发表时间：
2000-05-01
期刊：
影响因子：
7.2
通讯作者：
Shinozaki, K
中科院分区：
文献类型：
--
作者：
Ito, T;Kim, GT;Shinozaki, K
我们鉴定了一个与细胞质核糖体蛋白（RP） S13同源的基因（AtRPS13A）的解离（Ds）转座子插入拟南芥突变体。我们将突变体命名为尖首叶（pfl） 2，因为它的表型与RPS18基因的pfl1突变体相似。该突变体引起多种表型变化，包括叶片和毛状体形态异常，根系生长迟缓和开花延迟。显微分析表明，pfl2的第一片叶片中栅栏细胞数量明显减少，这表明突变表型是由细胞分裂减少引起的。然而，在生殖生长过程中未观察到表型变化。在拟南芥中，RPS13蛋白是由包括AtRPS13A在内的一个小表达基因家族编码的。pfl1 pfl2双突变体无加性效应。这些结果表明，RPS13在生长发育过程中具有定量和多效性的功能，由于其属于小基因家族，不同类型的RP基因位点的突变可积累而不会产生严重的生长缺陷。
We identified a Dissociation (Ds) transposon-inserted Arabidopsis mutant of a gene (AtRPS13A) homologous to cytoplasmic ribosomal protein (RP) S13. We named our mutant pointed first leaf (pfl) 2 because of its similar phenotype to the pfl1 mutant of the RPS18 gene. This mutant caused multiple phenotypic changes, including aberrant leaf and trichome morphology, retarded root growth, and late flowering. Microscopic analysis showed that the first leaf blade of pfl2 contained a significantly reduced number of palisade cells, which suggests that the mutant phenotype was caused by reduced cell division. However, no phenotypic changes were observed during reproductive growth. In Arabidopsis, the RPS13 protein was encoded by a small expressed gene family including AtRPS13A. A pfl1 pfl2 double mutant showed no additive effect. These results suggest that RPS13 functions in quantitative and pleiotropic ways during growth and development, and that mutations at different kinds of RP gene loci are accumulatable without serious growth defects because they belong to small gene families.