Electrospun UiO-66/polyacrylonitrile nanofibers as efficient sorbent for pipette tip solid phase extraction of phytohormones in vegetable samples
Electrospun UiO-66/polyacrylonitrile nanofibers as efficient sorbent for pipette tip solid phase extraction of phytohormones in vegetable samples
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静电纺丝 UiO-66/聚丙烯腈纳米纤维作为移液器吸头固相萃取蔬菜样品中植物激素的高效吸附剂
DOI：
10.1016/j.chroma.2018.02.030
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发表时间：
2018
影响因子：
4.1
通讯作者：
Pang Jie
中科院分区：
文献类型：
--
作者：
Yan Zhiming;Wu Mei;Hu Biqing;Yao Minna;Zhang Lan;Lu Qiaomei;Pang Jie
本文首次将金属-有机骨架颗粒复合纤维（UiO-66/PAN纳米纤维）作为吸附剂应用于吸头固相萃取（PT-SPE）中。采用静电纺丝法制备了UiO-66/PAN纳米纤维，并通过傅立叶变换红外光谱、扫描电镜、X射线衍射和氮气吸附-脱附实验对纤维进行了表征。将UiO-66/PAN纳米纤维组装成新型PT-SPE柱，用于高效液相色谱（HPLC）测定4种植物激素。研究了UiO-66/PAN纳米纤维的种类、用量、溶液pH值、离子强度和解吸条件等因素对UiO-66/PAN纳米纤维吸附性能的影响。在最佳条件下，植物激素的线性范围为0.06-60 ng/mL，相关系数大于0.992。检测限在0.01 ng/mL至0.02 ng/mL之间。四种植物激素（各15 ng/mL）的三次重复提取的日间和日间精密度（RSD）在1.5- 5.6%范围内。该方法已成功地应用于西瓜和绿豆芽中植物激素的测定。结果表明，荷正电的UiO-66与阴离子形式的植物激素之间的静电相互作用在植物激素的提取中起着重要作用。
In this work, metal-organic framework particles incorporated fibers (UiO-66/PAN nanofibers) were used as adsorbent in pipette tip solid phase extraction (PT-SPE) for the first time. The UiO-66/PAN nanofibers were fabricated by a facile electrospinning method and characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and nitrogen adsorption-desorption experiments. The UiO-66/PAN nanofibers were applied to assemble a novel PT-SPE cartridge for determination of four phytohormones followed by high performance liquid chromatography (HPLC). Several experimental parameters such as kinds of UiO-66/PAN nanofibers, the amount of UiO-66/PAN nanofibers, the effect of solution pH, ionic strength and desorption conditions were intensively investigated. Under the optimal conditions, the linear ranges of the phytohormones were in the range of 0.06–60 ng/mL with correlation coefficients above 0.992. The limits of detection were between 0.01 ng/mL to 0.02 ng/mL. The interday and intraday precision (RSD) for three replicate extractions of the four phytohormones (15 ng/mL for each) was in the range of 1.5–5.6%. The established method was successfully applied for the determination of phytohormones in watermelon and mung bean sprouts samples. The results showed that the electrostatic interaction between the positively charged UiO-66 and anionic forms of phytohormones played an important role in the extraction of the phytohormones.