PVDF Nanofiber Scaffold Coated with a Vitronectin Peptide Facilitates the Neural Differentiation of Human Embryonic Stem Cells.
PVDF Nanofiber Scaffold Coated with a Vitronectin Peptide Facilitates the Neural Differentiation of Human Embryonic Stem Cells.
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DOI：
10.12717/dr.2020.24.2.135
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发表时间：
2020-06-01
期刊：
影响因子：
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通讯作者：
Kim, Dae-Sung
中科院分区：
文献类型：
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作者：
Jeon, Byeong-Min;Yeon, Gyu-Bum;Kim, Dae-Sung
聚偏二氟乙烯（PVDF）是一种稳定的生物相容性材料，已广泛应用于生物医学领域。由于其压电特性，PVDF的静电纺丝膜已被用于培养电活性细胞，如骨细胞和心肌细胞。在这里，利用PVDF的压电性能，我们已经制作了PVDF的可移植支架使用静电纺丝技术分化人类胚胎干细胞（hESCs）成神经前体细胞（NP）。用来自玻连蛋白的肽进行表面包被使得hESC能够牢固地粘附到支架上，并在双SMAD抑制下分化成NP。与Matrigel相比，我们的支架更显著地支持hESC分化为SOX 1阳性NP。纳米颗粒在支架上的生长可以促进神经元、星形胶质细胞和少突胶质细胞前体的形成。此外，比较转录组分析揭示了27个基因的可变表达的支架组，其中几个是高度相关的神经分化所需的生物过程。这些结果表明，用玻连蛋白肽包被的PVDF支架可以作为hESC神经分化的高效和确定的培养平台。
Polyvinylidene fluoride (PVDF) is a stable and biocompatible material that has been broadly used in biomedical applications. Due to its piezoelectric property, the electrospun nanofiber of PVDF has been used to culture electroactive cells, such as osteocytes and cardiomyocytes. Here, taking advantage of the piezoelectric property of PVDF, we have fabricated a PVDF nanofiber scaffolds using an electrospinning technique for differentiating human embryonic stem cells (hESCs) into neural precursors (NPs). Surface coating with a peptide derived from vitronectin enables hESCs to firmly adhere onto the nanofiber scaffolds and differentiate into NPs under dual-SMAD inhibition. Our nanofiber scaffolds supported the differentiation of hESCs into SOX1-positive NPs more significantly than Matrigel. The NPs generated on the nanofiber scaffolds could give rise to neurons, astrocytes, and oligodendrocyte precursors. Furthermore, comparative transcriptome analysis revealed the variable expressions of 27 genes in the nanofiber scaffold groups, several of which are highly related to the biological processes required for neural differentiation. These results suggest that a PVDF nanofiber scaffold coated with a vitronectin peptide can serve as a highly efficient and defined culture platform for the neural differentiation of hESCs.