Proofs of the structure of lipid coated nanoparticles (SMBV™) used as drug carriers
Proofs of the structure of lipid coated nanoparticles (SMBV™) used as drug carriers
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DOI：
10.1023/a:1007504124603
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发表时间：
2000-07-01
影响因子：
3.7
通讯作者：
Betbeder, D
中科院分区：
文献类型：
--
作者：
De Miguel, I;Imbertie, L;Betbeder, D
目的。超分子生物载体（SMBV(TM)）由被脂质膜包围的交联阳离子纳米颗粒组成。目的是研究脂质膜的结构及其与纳米颗粒的相互作用，以区分SMBV（TM）与其他聚合物/脂质结合物。利用zeta电位、荧光能量转移（FET）和荧光显微镜研究了脂质与纳米颗粒表面的相互作用。将SMBV（TM）与脂质体及纳米颗粒/脂质体混合物进行了比较。最后用电镜观察了SMBV（TM）的结构。Zeta电位测量表明，SMBV（TM）表面的脂质对表面电荷有明显的屏蔽作用。而纳米颗粒和脂质体的混合物则不是这样。FET实验证实了这些结果，表明SMBV（TM）的脂质更接近纳米颗粒表面。SMBV（TM）模型微颗粒的荧光显微镜显示SMBV（TM）表面有一个脂质冠，在SMBV（TM）纳米颗粒上的电子显微镜也证实了这一点。结果表明，在SMBV（TM）情况下，脂质在多糖核表面被强烈吸附，这可能是由于离子/疏水相互作用所致。由此产生的超分子结构是由磷脂/胆固醇层包围的球形阳离子多糖颗粒。
Purpose. Supramolecular Biovectors (SMBV(TM)) consist of cross-linked cationic nanoparticles surrounded by a lipid membrane. The purpose was to study the structure of the lipid membrane and to characterise its interaction with the nanoparticles in order to differentiate SMBV(TM) from other polymer/lipid associations.Methods. The interaction of lipids with the nanoparticle surface was studied using zeta potential, Fluorescence Energy Transfer (FET) and Fluorescence Microscopy. SMBV(TM) were compared to liposomes and mixtures nanoparticles/liposomes. Finally the structure of SMBV(TM) was visualised by Electron Microscopy.Results. Zeta potential measurements showed that lipids on SMBV(TM) had a pronounced shielding effect on the surface charge. This was not the case for mixtures of nanoparticles and liposomes. FET experiments confirmed these results indicating that, for SMBV(TM), the lipids are much closer to the nanoparticle surface. SMBV(TM) Fluorescence microscopy on model microparticles showed a lipid crown on SMBV(TM) that was confirmed by electron microscopy on SMBV(TM) nanoparticles.Conclusions. Results show that in case of SMBV(TM) lipids are strongly adsorbed on the polysaccharide core surface probably due to ionic/hydrophobic interactions. The resulting supramolecular structure is a spherical cationic polysaccharide particle surrounded by a phospholipid/cholesterol layer.