Jasmonate complements the function of Arabidopsis lipoxygenase3 in salinity stress response
Jasmonate complements the function of Arabidopsis lipoxygenase3 in salinity stress response
复制标题
茉莉酸可补充拟南芥脂氧合酶 3 在盐度胁迫反应中的功能。
DOI：
10.1016/j.plantsci.2015.11.009
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发表时间：
2016-03-01
期刊：
影响因子：
5.2
通讯作者：
Yang, Chengwei
中科院分区：
文献类型：
--
作者：
Ding, Hui;Lai, Jianbin;Yang, Chengwei
茉莉酸AM在各种胁迫反应中的作用已被详细揭示，但其在耐盐性中的作用尚不清楚。在这里，我们研究了与茉莉酸合成有关的拟南芥脂肪氧合酶3(LOX3)在盐胁迫反应中的功能。转录分析表明，LOX3在盐处理下被显著诱导。与野生型相比，LOX3突变体在萌发和不同发育阶段均表现出对盐胁迫的敏感。有趣的是，茉莉酸甲酯(MeJA)挽救了LOX3突变体的盐敏表型，表明该突变体的盐度反应受损可能是由JA介导的。此外，LOX3突变体的侧根数在正常条件下与野生型相似，但在盐处理过程中低于野生型，而且这种侧根敏感表型也得到了外源MeJA的补充。此外，LOX3突变体中氧化脂质的测定和JA受体线圈突变体在盐胁迫下的萌发分析支持JA可能调节对盐胁迫的早期反应。综上所述，我们研究了LOX3在盐胁迫反应中的新功能，发现突变的LOX3对盐的敏感性可以被MeJA互补，为JA与耐盐性之间的关联提供了新的证据。(C)2015爱思唯尔爱尔兰有限公司。保留所有权利。
The functions of jasmonic acid am in various stress responses have been uncovered in details, but its role in salt tolerance remains unclear. Here, we characterize the function of Arabidopsis lipoxygenase3 (LOX3), an enzyme involved in JA synthesis, in salt stress response. The transcriptional analysis indicated that LOX3 was dramatically induced under salt treatment. Compared with wild type, the lox3 mutant exhibited hypersensitivity to salt stress in germination and different developmental stages. Interestingly, methyl jasmonate (MeJA) rescued the salt sensitivity phenotypes of the lox3 mutant, suggesting the impairment of salinity response in the mutant may be mediated by JA. Furthermore, the lateral root number of the lox3 mutant was similar with that in wild type under normal condition, but less than that in wild type during salt treatment, and this lateral root sensitivity phenotype was also complemented by exogenous MeJA. In addition, the measurement of oxylipins in the lox3 mutant and the analysis on germination of the JA receptor coil mutant under salt stress supported that JA may regulate the early response to salinity. In conclusion, we characterized the novel function of LOX3 in salinity stress response, and found that the salt hypersensitivity of the lox3 mutant can be complemented by MeJA, providing new evidence for the association between JA and salt tolerance. (C) 2015 Elsevier Ireland Ltd. All rights reserved.