Specific reversal of cytolytic T cell-target cell functional binding is induced by free target cells.
Specific reversal of cytolytic T cell-target cell functional binding is induced by free target cells.
复制标题
游离靶细胞诱导溶细胞性 T 细胞与靶细胞功能结合的特异性逆转。
DOI：
10.4049/jimmunol.127.1.51
复制
发表时间：
1981
影响因子：
4.4
通讯作者：
M. Mescher
中科院分区：
文献类型：
--
作者：
Steven P. Balk;M. Mescher
通过使用一种针对特异性靶细胞结合的功能测定法，对同种异体细胞毒性T淋巴细胞（CTL）与51铬标记的靶细胞之间特定细胞 - 细胞黏附的逆转情况进行了研究。CTL与肿瘤细胞靶标P815肥大细胞瘤之间的功能性黏附在悬浮液中能稳定维持5小时以上。然而，加入未标记的靶细胞会导致功能性结合迅速逆转。这种逆转似乎具有特异性，因为其他H - 2型肿瘤细胞不会诱导逆转。在存在未标记的P815细胞或合适H - 2型的正常脾细胞时，CTL与同基因于P815的标记脾细胞之间的结合物也会发生功能性逆转。P815诱导的逆转效率明显高于正常脾细胞。此外，合适H - 2型的正常脾细胞不会诱导CTL - P815靶细胞结合物的逆转。从P815细胞纯化得到的质膜不会特异性地逆转CTL与P815或正常脾细胞靶标的功能性结合。这些结果表明，功能性CTL - 靶细胞结合可通过与游离靶细胞相互作用而特异性逆转。逆转速率似乎取决于CTL对结合态与游离态靶细胞的相对亲和力。CTL可能为研究特定细胞 - 细胞黏附的逆转提供一个有用的系统。
Reversal of specific cell-cell adhesions between allogeneic cytolytic T lymphocytes (CTL) and 51Cr-labeled target cells has been studied by using a functional assay for specific target cell binding. Functional adhesions between CTL and a tumor cell target, the P815 mastocytoma, were stable in suspension for greater than 5 hr. Addition of unlabeled target cells, however, resulted in rapid reversal of functional binding. This reversal appeared to be specific in that no reversal was induced by tumor cells of other H-2 types. Functional reversal of conjugates between CTL and labeled spleen cells syngeneic with P815 also occurred in the presence of unlabeled P815 cells or normal spleen cells of the appropriate H-2 type. The reversal by P815 was substantially more efficient than by normal spleen cells. Furthermore, normal spleen cells of the appropriate H-2 type did not induce reversal of CTL-P815 target conjugates. Purified plasma membranes from P815 cells did not specifically reverse functional binding of CTL to either P815 or normal spleen cell targets. These results demonstrate that functional CTL-target cell binding can be specifically reversed by interaction with free target cells. The rate of reversal appears to be dependent on the relative affinity of the CTL for the bound versus the free target cell. CTL may provide a useful system for studying reversal of specific cell-cell adhesions.