A sensitive and quantitative element-tagged immunoassay with ICPMS detection
A sensitive and quantitative element-tagged immunoassay with ICPMS detection
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DOI：
10.1021/ac0110350
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发表时间：
2002-04-01
影响因子：
7.4
通讯作者：
Tanner, SD
中科院分区：
文献类型：
--
作者：
Baranov, VI;Quinn, Z;Tanner, SD
我们报告了一套新型免疫测定方法，在这种方法中，可以使用特定元素标记抗体检测相关蛋白质。这些免疫测定与电感耦合等离子体质谱仪（ICPMS）直接耦合，以量化反应标记抗体中的元素（在本研究中为金属）成分。实验证明，这些方法可以检测低至 0.1 -0.5 纳克/毫升的目标蛋白质水平，并对蛋白质浓度产生超过 3 个数量级的线性响应。
We report a set of novel immunoassays in which proteins of interest can be detected using specific element-tagged antibodies. These immunoassays are directly coupled with an inductively coupled plasma mass spectrometer (ICPMS) to quantify the elemental (in this work, metal) component of the reacted tagged antibodies. It is demonstrated that these methods can detect levels of target proteins as low as 0.1 -0.5 ng/mL and yield a linear response to protein concentration over 3 orders of magnitude.