REINNERVATION OF TRANSPLANTED PANCREATIC-ISLETS - A COMPARISON AMONG ISLETS IMPLANTED INTO THE KIDNEY, SPLEEN, AND LIVER
REINNERVATION OF TRANSPLANTED PANCREATIC-ISLETS - A COMPARISON AMONG ISLETS IMPLANTED INTO THE KIDNEY, SPLEEN, AND LIVER
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DOI：
10.1097/00007890-199307000-00026
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发表时间：
1993-07-01
期刊：
影响因子：
6.2
通讯作者：
SUNDLER, F
中科院分区：
文献类型：
--
作者：
KORSGREN, O;JANSSON, L;SUNDLER, F
移植到肾包膜下的胰岛在植入后的前3-4个月内通过传入和传出神经纤维进行神经再支配。为了评价移植器官在这一过程中的重要性，本研究比较了移植到肝、肾和脾中的胰岛神经纤维再支配的程度和类型。为此，将150个同系胰岛移植到C57 BL/6小鼠的肾包膜下。此外，将150个胰岛注射到相同动物的脾脏或肝脏中。所有动物在移植后14周处死，之后对移植器官进行神经肽和酪氨酸羟化酶（TH）的间接免疫荧光处理，并用乙酰胆碱酯酶（AchE）染色以观察神经纤维。两个传入（含有P物质和/或降钙素基因相关肽）和副交感神经（含有血管活性肠肽或乙酰胆碱酯酶）神经纤维的情况下，从植入到脾脏的胰岛，偶尔CGRP纤维被认为是植入到肝脏的胰岛，和所有这些纤维经常被认为是在肾包膜下植入的胰岛。植入肝脏或脾脏的胰岛含有密集的交感神经（含有神经肽Y和TH）神经纤维网络，其密度通常高于肾包膜下的胰岛移植物。然而，植入肝脏的胰岛中有五分之一完全没有明显的神经纤维。总之，移植到不同植入部位的胰岛的神经再支配模式存在显著差异。
Islets transplanted beneath the kidney capsule become reinnervated during the first 3-4 months after implantation by both afferent and efferent nerve fibers. To evaluate the importance of the implantation organ for this process, the present study compared both the degree and the types of nerve fibers reinnervating islets transplanted into the liver, kidney, and spleen. For this purpose, 150 syngeneic islets were grafted under the kidney capsule of C57BL/6 mice. In addition, the same animals were injected with 150 islets into the spleen or liver. All animals were killed 14 weeks after transplantation, after which the graft-bearing organs were processed for indirect immunofluorescence for neuropeptides and tyrosine hydroxylase (TH), and with acetyl cholinesterase (AchE) staining to visualize nerve fibers. Both afferent (containing substance P and/or calcitonin gene-related peptide) and parasympathetic (containing vasoactive intestinal peptide or AchE) nerve fibers were absent from islets implanted into the spleen; an occasional CGRP fiber was seen in islets implanted into the liver; and all these fibers were regularly seen in islets implanted beneath the renal capsule. The islets implanted into the liver or spleen contained a dense network of sympathetic (containing neuropeptide Y and TH) nerve fibers that was often more dense than in the islet grafts under the kidney capsule. One-fifth of islets implanted into the liver were, however, completely devoid of demonstrable nerve fibers. In conclusion, there are marked differences with regard to the pattern of reinnervation of islets transplanted to different implantation sites.