Gene amplification as a developmental strategy:: Isolation of two developmental amplicons in Drosophila
Gene amplification as a developmental strategy:: Isolation of two developmental amplicons in Drosophila
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DOI：
10.1016/s1534-5807(03)00398-8
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发表时间：
2004-01-01
影响因子：
11.8
通讯作者：
Orr-Weaver, TL
中科院分区：
文献类型：
--
作者：
Claycomb, JM;Benasutti, M;Orr-Weaver, TL
众所周知，基因扩增在少数情况下对上调基因表达至关重要，但扩增在不同生物体发育中的应用程度尚不清楚。通过将基因组DNA定量杂交到微阵列；为了测定基因拷贝数，我们在果蝇卵巢的卵泡细胞中鉴定了另外两个发育扩增片段。这两个扩增子都含有基因，这些基因在扩增后在毛囊细胞中表达，其中三个基因的表达限制在分化后期的特化毛囊细胞。遗传分析证实，这些基因中至少有一个黄-g基因对毛囊细胞功能至关重要，因为黄-g基因的突变会破坏蛋壳的完整性。因此，在毛囊细胞分化期间，随着细胞变得多倍体，整个基因组被过度复制，随后特定的基因组间隔被过度复制以促进基因表达。
Gene amplification is known to be critical for upregulating gene expression in a few cases, but the extent to which amplification is utilized in the development of diverse organisms remains unknown. By quantifying genomic DNA hybridization to microarrays; to assay gene copy number, we identified two additional developmental amplicons in the follicle cells of the Drosophila ovary. Both amplicons contain genes which, following their amplification, are expressed in the follicle cells, and the expression of three of these genes becomes restricted to specialized follicle cells late in differentiation. Genetic analysis establishes that at least one of these genes, yellow-g, is critical for follicle cell function, because mutations in yellow-g disrupt eggshell integrity. Thus, during follicle cell differentiation the entire genome is overreplicated as the cells become polyploid, and subsequently specific genomic intervals are overreplicated to facilitate gene expression.