A resolution measure for three-dimensional microscopy
A resolution measure for three-dimensional microscopy
复制标题
DOI：
10.1016/j.optcom.2009.01.062
复制
发表时间：
2009-05-01
影响因子：
2.4
通讯作者：
Ober, Raimund J.
中科院分区：
文献类型：
--
作者：
Chao, Jerry;Ram, Sripad;Ober, Raimund J.
介绍了一种用于常规光学显微镜的三维(3D)分辨率测量方法，它克服了经典的3D(轴)分辨率限制的缺点。该3D分辨率测量是在参数估计问题的背景下并基于Cramer-Rao下限而形成的，该3D分辨率测量指示可以从获取的图像确定3D空间中两个对象之间的给定距离的精度。它预测，在来自感兴趣物体的足够多的光子的情况下，可以以预先指定的精度估计任意小的间隔距离。利用点源对的模拟图像，我们证明了最大似然估计器能够达到分辨率测量所预测的精度。我们还演示了不同的因素，如外部噪声源和成像对象对的空间方向，如何影响确定给定距离的精度。(C)2009爱思唯尔B.V.保留所有权利。
A three-dimensional (3D) resolution measure for the conventional optical microscope is introduced which overcomes the drawbacks of the classical 3D (axial) resolution limit. Formulated within the context of a parameter estimation problem and based on the Cramer-Rao lower bound, this 3D resolution measure indicates the accuracy with which a given distance between two objects in 3D space can be determined from the acquired image. It predicts that, given enough photons from the objects of interest, arbitrarily small distances of separation can be estimated with prespecified accuracy. Using simulated images of point source pairs, we show that the maximum likelihood estimator is capable of attaining the accuracy predicted by the resolution measure. We also demonstrate how different factors, such as extraneous noise sources and the spatial orientation of the imaged object pair, can affect the accuracy with which a given distance of separation can be determined. (C) 2009 Elsevier B.V. All rights reserved.