Von Willebrand protein binds to extracellular matrices independently of collagen.
Von Willebrand protein binds to extracellular matrices independently of collagen.
复制标题
血管性血友病蛋白独立于胶原蛋白与细胞外基质结合。
DOI：
10.1073/pnas.81.2.471
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发表时间：
1984
影响因子：
11.1
通讯作者：
Marder,VJ
中科院分区：
文献类型：
--
作者：
Wagner,DD;Urban-Pickering,M;Marder,VJ
血管性血友病蛋白存在于内皮细胞的细胞外基质中，与纤维连接蛋白和IV型和V型胶原共分布。细菌胶原酶消化内皮细胞去除纤维性胶原蛋白，但纤维连接蛋白和血管性血液病蛋白的模式保持不变。外源性血管性血友病蛋白与不同细胞基质结合，无论胶原蛋白丰富与否。血管性血液病蛋白也可以修饰存在于α， α '-二吡啶（一种胶原分泌抑制剂）的细胞基质。这些结果表明，血管性血友病蛋白存在于内皮细胞的细胞外基质中，并且血管性血友病蛋白与外源基质的结合不依赖于基质胶原。
Von Willebrand protein is present in the extracellular matrix of endothelial cells where it codistributes with fibronectin and types IV and V collagen. Bacterial collagenase digestion of endothelial cells removed fibrillar collagen, but the pattern of fibronectin and of von Willebrand protein remained undisturbed. Exogenous von Willebrand protein bound to matrices of different cells, whether rich or poor in collagen. von Willebrand protein also decorated the matrix of cells grown in the presence of alpha, alpha'-dipyridyl, a collagen-secretion inhibitor. These results indicate that the presence of von Willebrand protein in the extracellular matrix of endothelial cells and the binding of von Willebrand protein to foreign matrices do not depend on the matrix collagen.