Quantitative Experimental Embryology: A Modern Classical Approach.

Quantitative Experimental Embryology: A Modern Classical Approach.
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DOI:
10.3390/jdb10040044
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发表时间:
2022-10-18
影响因子:
2.7
通讯作者:
Steventon B
Steventon B
中科院分区:
其他
文献类型:
--
作者:
Busby L;Saunders D;Nájera GS;Steventon B

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实验胚胎学通常被称为发育生物学的经典方法,在一定程度上被分子生物学和遗传技术的引入所取代。这种方法与先进的技术相结合,揭示神经嵴发育的核心原则由罗伯托市长的实验室的启发,我们回顾了实验胚胎学的关键定量的例子,从最近的工作在广泛的发育生物学问题。我们建议,定量实验胚胎学提供了必要的方法来探索细胞和组织的反应,靶向细胞的添加,去除和限制。在这样做的过程中,它是一种重要的方法,可以揭示仍然难以捉摸的开发原则,例如模式调节,缩放和自组织。
Experimental Embryology is often referred to as a classical approach of developmental biology that has been to some extent replaced by the introduction of molecular biology and genetic techniques to the field. Inspired by the combination of this approach with advanced techniques to uncover core principles of neural crest development by the laboratory of Roberto Mayor, we review key quantitative examples of experimental embryology from recent work in a broad range of developmental biology questions. We propose that quantitative experimental embryology offers essential ways to explore the reaction of cells and tissues to targeted cell addition, removal, and confinement. In doing so, it is an essential methodology to uncover principles of development that remain elusive such as pattern regulation, scaling, and self-organisation.
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