OsPS6 Plays Important Role in Anther Development and Microspore Formation
OsPS6 Plays Important Role in Anther Development and Microspore Formation
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DOI：
10.1016/j.rsci.2020.05.002
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发表时间：
2020-03
期刊：
影响因子：
4.8
通讯作者：
Zhou Weimin;MA Menghui;Sun Lianping;Z. Peng;LV Shiqiong;Zhong Zheng-zheng;Tong Hanhua
中科院分区：
文献类型：
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作者：
Zhou Weimin;MA Menghui;Sun Lianping;Z. Peng;LV Shiqiong;Zhong Zheng-zheng;Tong Hanhua
在开花植物中，二倍体孢子体和单倍体配子体世代之间的交替生活史是通过孢子体和配子体的协同作用完成的。花药腔中有活力的花粉粒的形成是该过程的关键（Scott et al，2004; Ma，2005; Wilson and Zhang，2009）。四层花药壁在整个过程中具有特定的功能和协调作用，在花粉粒的形成中起着重要作用（Walbot和Egger，2016）。在花粉成熟阶段，中层和绒毡层经历程序性退化（Scott et al，2004）。花药壁最内层的绒毡层与发育中的小孢子直接接触（教区and Li，2010）。绒毡层细胞为花粉壁的正常发育提供营养和其他化学物质，包括孢粉素前体。在水稻中，多个基因在花药层退化过程中起着重要作用，包括多孢子细胞1（MSP 1）、绒毡层退化阻滞（TDR）、绒毡层退化阻滞相互作用蛋白2（TDR-interacting protein 2（TIP 2，bHLH 142），成髓细胞瘤（MYB）转录因子GAMYB，植物同源结构域（PHD）-指蛋白持续绒毡层细胞1（PTC 1）和TGACG基序结合因子（TGA）转录因子OsTGA 10（Zhao等人，2002; Nonomura等人，2003; Jung等人，2005; Li等人，2006; Aya等人，2009; Fu等人，2014; Ko等人，2014; Ranjan等人，2017; Chen等人，2018; Du等人，2019）。因此，获得由新基因或复等位基因控制的花粉不育突变体对研究雄性不育机理具有重要意义。为了筛选水稻小孢子形成和花药发育的关键基因，以日本晴为材料构建了水稻突变体文库。SSP. japonica）通过用甲基磺酸乙酯（EMS）处理。在该群体中鉴定出一个完全雄性不育突变体osps 6。在
In flowering plants, the alternating life cycle between diploid sporophyte and haploid gametophyte generations is completed through the synergistic action of sporophyte and gametophyte. The formation of viable pollen grains in anther cavity is the key to the process (Scott et al, 2004; Ma, 2005; Wilson and Zhang, 2009). The four-layer anther wall has specific functions and coordination in the whole process, and plays an important role in the formation of pollen grains (Walbot and Egger, 2016). During the pollen maturity stage, the middle layer and the tapetum undergo programmed degeneration (Scott et al, 2004). The tapetum which is the innermost layer of anther wall directly contacts with the developing microspores (Parish and Li, 2010). Tapetal cells may support nutrients and other chemicals including sporopollenin precursors for the normal development of pollen wall. In rice, multiple genes play essential roles in the process of the anther layers’ degeneration, including MULTIPLE SPOROCYTE1 (MSP1), UNDEVELOPED TAPETUM (UDT1), tapetum degeneration retardation (TDR), TDR-interacting protein 2 (TIP2, bHLH142), the myeloblastosis (MYB) transcription factor GAMYB, plant homeodomain (PHD)-finger protein persistent tapetal cell 1 (PTC1) and TGACG MOTIF-BINDING FACTOR (TGA) transcription factor OsTGA10 (Zhao et al, 2002; Nonomura et al, 2003; Jung et al, 2005; Li et al, 2006; Aya et al, 2009; Fu et al, 2014; Ko et al, 2014; Ranjan et al, 2017; Chen et al, 2018; Du et al, 2019). Therefore, it is important to obtain pollen sterile mutants controlled by new genes or multiple alleles for studying the mechanism of male sterility. To identify important genes critical for microspore formation and anther development in rice, a rice mutant library was generated using Nipponbare (Oryza sativa L. ssp. japonica) by treatment with ethylmethane sulfonate (EMS). A complete male sterility mutant osps6 was identified in this population. At the