Target-driven rolling walker based electrochemical biosensor for ultrasensitive detection of circulating tumor DNA using doxorubicin@tetrahedron-Au tags
Target-driven rolling walker based electrochemical biosensor for ultrasensitive detection of circulating tumor DNA using doxorubicin@tetrahedron-Au tags
复制标题
基于目标驱动的滚动步行器的电化学生物传感器，使用阿霉素@四面体-Au 标签超灵敏地检测循环肿瘤 DNA
DOI：
10.1016/j.bios.2019.111826
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发表时间：
2020-01-15
影响因子：
12.6
通讯作者：
Chen, Weixian
中科院分区：
文献类型：
--
作者：
Li, Dandan;Xu, Yixin;Chen, Weixian
本研究以多柔比星@四面体金（DOX@TDN-Au）为电化学指示剂，通过滚环扩增（RCA）技术，建立了一种多腿、高度集成的DNA滚动步行者电化学生物传感器。在靶驱动RCA后，多腿步行者可以通过链置换反应沿着预先设计的轨道沿着移动，导致多条腿不可逆地结合iStep探针。iStep探针与质量腿的结合可以有效地阻止DOX@TDN-Au标签与传感器表面的结合，从而达到“信号关闭”状态。得益于滚步行者机器和DOX@TDN-Au标签的高放大效率，所建立的生物传感器对目标检测具有高灵敏度，检测下限低至0.29 fM。此外，目标ctDNA可以同时与环和捕获探针杂交，大大增强了所开发的生物传感方法的特异性。因此，这种生物传感方法是一种很有前途的工具，用于检测ctDNA在临床诊断和肿瘤进展评估领域。
In this study, a multiple-legged and highly integrated DNA rolling walker based electrochemical biosensor was developed for ultrasensitive ctDNA analysis through rolling circle amplification (RCA) with doxorubicin@tetrahedron-Au (DOX@TDN-Au) as electrochemical indicator. Upon target-driven RCA, the multiple-legged walker could move along with the predesigned track by strand displacement reactions, resulting in numbers of legs binding irreversibly to iStep probes. The binding of massive legs to iStep probes could effectively impede DOX@TDN-Au tags binding on the surface of sensor and then reached a "signal off" state. Benefiting from the highly amplified efficiency of rolling walker machine and DOX@TDN-Au tags, the established biosensor performed high sensitivity for target detection with a low limit of detection down to 0.29 fM. Moreover, the target ctDNA could hybridize with the ring and capture probe simultaneously, greatly enhancing the specificity of the developed biosensing method. Thus, this biosensing method is a promising tool for detection of ctDNA in the field of clinical diagnostic and tumor progression assessment.