The role of apoptosis in the early corneal wound healing after excimer laser keratectomy in the rat
The role of apoptosis in the early corneal wound healing after excimer laser keratectomy in the rat
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DOI：
10.1007/s004170000182
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发表时间：
2000-10-01
影响因子：
2.7
通讯作者：
O'Brien, TP
中科院分区：
文献类型：
--
作者：
Li, Q;Ashraf, MF;O'Brien, TP
背景：在大鼠模型中研究了细胞凋亡在准分子激光角膜切削术后角膜伤口愈合中的潜在作用。方法：刘易斯大鼠采用193 nm准分子激光进行激光角膜切削术。将中央角膜分为三个深度：A组，上皮; B组，浅层基质; C组，深层基质。在1、12、24和36 h以及1周时收集眼睛。通过免疫组织化学检测与凋亡相关的细胞标志物Fas、Fas配体（FasL）、Bcl-2和Bax。组织学评价角膜细胞耗竭和内皮变化。双链DNA断裂的原位末端标记用于证明角膜切片中的细胞凋亡。结果如下：在激光手术后12 h，12只大鼠中的6只（50%）（A、B和C组的总数）、24 h时15只大鼠中的11只（73%）、36 h时15只大鼠中的3只（20%）和1周时13只大鼠中的2只（15%）观察到角膜细胞耗竭。在消融区观察到角膜内皮水肿。Fas、FasL、Bcl-2和Bax在角膜细胞中的表达表现出与角膜细胞耗竭和内皮变化相似的动态变化。间质消融组新生上皮细胞凋亡分子表达较少，内皮细胞凋亡分子表达较多。结论：准分子激光角膜切削术可引起角膜基质细胞和内皮细胞凋亡。间质消融组的内皮水肿多于上皮消融组。凋亡分子的表达与角膜细胞的耗竭和再生以及内皮细胞的恢复相一致，表明细胞凋亡是准分子激光角膜切削术后角膜伤口愈合和重塑的动态部分。
Background: The potential role of apoptosis in corneal wound healing after excimer laser keratectomy was investigated in a rat model. Methods: Lewis rats underwent laser keratectomy using a 193-nm excimer laser. The central corneas were ablated in three depths: group A, epithelium; group B, superficial stroma; group C, deep stroma. Eyes were collected at 1, 12, 24, and 36 h and I week. Cellular markers associated with apoptosis - Fas, Fas ligand (FasL), Bcl-2, and Bax - were examined by immunohistochemistry. Keratocyte depletion and endothelial changes were evaluated histologically. In situ end labeling of double-stranded DNA breaks was used to demonstrate apoptosis in corneal sections. Results: Keratocyte depletion was observed in 6 (50%) of 12 rats (total from groups A, B, and C) at 12 h, 11 (73%) of 15 at 24 h, 3 (20%) of 15 at 36 h, and 2 (15%) of 13 at 1 week after laser surgery. Corneal endothelial edema was observed in the ablation zone. Expression of Fas, FasL, Bcl-2, and Bax in corneal cells showed dynamics similar to that of keratocyte depletion and endothelial changes. There was less expression of apoptotic molecules in newly generated epithelial cells and more in endothelial cells of the stromal ablation groups. Conclusions: Excimer laser keratectomy triggered apoptosis of corneal keratocytes and endothelial cells. More endothelial edema was observed in the stromal ablation than in the epithelial ablation group. The expression of apoptotic molecules coincided with the period of keratocyte depletion and regeneration and of endothelial recovery, suggesting that apoptosis is a dynamic part of corneal wound healing and remodeling after excimer laser keratectomy.