Reduced hemodialysis-induced oxidative stress in end-stage renal disease patients by electrolyzed reduced water
Reduced hemodialysis-induced oxidative stress in end-stage renal disease patients by electrolyzed reduced water
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DOI：
10.1046/j.1523-1755.2003.00118.x
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发表时间：
2003-08-01
影响因子：
19.6
通讯作者：
Chien, CT
中科院分区：
文献类型：
--
作者：
Huang, KC;Yang, CC;Chien, CT
背景终末期肾病（ESRD）患者的氧化应激增加可能会氧化大分子，从而导致慢性血液透析期间的心血管事件。电解还原水（ERW）具有清除活性氧（ROS）的能力，可能对降低终末期肾病患者血液透析诱导的氧化应激具有潜在作用。我们开发了一种化学发光发射光谱和高效液相色谱分析，以评估ERW替代对接受血液透析的ESRD患者血浆ROS（H-2 O-2和HOCl）清除活性和氧化脂质或蛋白质产生的影响。检测氧化标志物双酪氨酸、甲基胍、磷脂酰胆碱氢过氧化物，炎症标志物白细胞介素6（IL-6）、C反应蛋白（CRP）。虽然血液透析有效地去除了二酪氨酸和肌酐，但血液透析增加了氧化应激，包括磷脂酰胆碱氢过氧化物和甲基胍。血液透析降低了血浆ROS清除活性，如参考H-2 O-2和HOCl计数（分别为Rh-2 o（2）和Rhocl）增加和抗氧化活性降低（在本研究中表示为总抗氧化状态）所示。在1个月的治疗期间，ERW治疗减少了血液透析增强的Rh-2 o（2）和Rhocl，最大限度地减少了氧化和炎症标志物（CRP和IL-6），并部分恢复了总抗氧化状态。这项研究表明，血液透析与ERW管理可以有效地提高H-2 O-2 -和HOCl依赖的抗氧化防御和减少H-2 O-2 -和HOCl诱导的氧化应激。
Background. Increased oxidative stress in end-stage renal disease (ESRD) patients may oxidize macromolecules and consequently lead to cardiovascular events during chronic hemodialysis. Electrolyzed reduced water (ERW) with reactive oxygen species (ROS) scavenging ability may have a potential effect on reduction of hemodialysis-induced oxidative stress in ESRD patients.Methods. We developed a chemiluminescence emission spectrum and high-performance liquid chromatography analysis to assess the effect of ERW replacement on plasma ROS (H-2 O-2 and HOCl) scavenging activity and oxidized lipid or protein production in ESRD patients undergoing hemodialysis. Oxidized markers, dityrosine, methylguanidine, and phosphatidylcholine hydroperoxide, and inflammatory markers, interleukin 6 (IL-6), and C-reactive protein (CRP) were determined.Results. Although hemodialysis efficiently removes dityrosine and creatinine, hemodialysis increased oxidative stress, including phosphatidylcholine hydroperoxide, and methylguanidine. Hemodialysis reduced the plasma ROS scavenging activity, as shown by the augmented reference H-2 O-2 and HOCl counts (Rh-2 o(2) and Rhocl, respectively) and decreased antioxidative activity (expressed as total antioxidant status in this study). ERW administration diminished hemodialyis-enhanced Rh-2 o(2) and Rhocl, minimized oxidized and inflammatory markers (CRP and IL-6), and partly restored total antioxidant status during 1-month treatment.Conclusion. This study demonstrates that hemodialysis with ERW administration may efficiently increase the H-2 O-2 - and HOCl-dependent antioxidant defense and reduce H-2 O-2 - and HOCl-induced oxidative stress.