Complete genome and phylogenetic position of bovine papillomavirus type 7
Complete genome and phylogenetic position of bovine papillomavirus type 7
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DOI：
10.1099/vir.0.82794-0
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发表时间：
2007-07-01
影响因子：
3.8
通讯作者：
Shirasawa, Hiroshi
中科院分区：
文献类型：
--
作者：
Ogawa, Tornoko;Tomita, Yoshimi;Shirasawa, Hiroshi
牛乳头状病毒（BPV）有6个型别和16个推定型别。在这里，完整的基因组序列的BAPV 6，一种新的推定的BPV类型，从牛在日本分离，确定通过使用多重引物滚环扩增。该基因组全长7412 bp（G + C含量为46 mol%），编码5个早期基因（E1、E2、E4、E6和E7）和2个晚期基因（L1和L2），但不编码E5基因。E6蛋白含有非共有CxxC（x）（33）CxxC和共有CxxC（x）（29）CxxC锌结合结构域，E7蛋白缺乏LxCxE基序。L1开放阅读框（ORF）的核苷酸序列与β乳头瘤病毒属、γ乳头瘤病毒属和乳头瘤病毒属成员的L1 ORF最密切相关（57- 58%）。基于完整的L1 ORF的系统发育分析表明，BAPV 6应该被分类在一个新的属在家庭乳头瘤病毒BPV-7。
Six bovine papillonnavirus (BPV) types and 16 putative BPV types have been reported previously. Here, the complete genome sequence of BAPV6, a novel putative BPV type isolated from cattle in Japan, was determined by using multiple-primed rolling-circle amplification. The genome consisted of 7412 bp (G + C content of 46 mol%) that encoded five early (E1, E2, E4, E6 and E7) and two late (L1 and L2) genes, but did not encode the E5 gene. The E6 protein contained a non-consensus CxxC(x)(33)CxxC and a consensus CxxC(x)(29)CxxC zinc-binding domain, and the E7 protein lacked the LxCxE motif. The nucleotide sequence of the L1 open reading frame (ORF) was related most closely (57-58 %) to the L1 ORF of member(s) of the genera Betapapillomavirus, Gammapapillormvirus and Pipapillomavirus. Phylogenetic analysis based on the complete L1 ORF suggests that BAPV6 should be classified in a novel genus in the family Papillomaviridae as BPV-7.