Comparison of photopolymerizable thiol-ene PEG and acrylate-based PEG hydrogels for cartilage development.
Comparison of photopolymerizable thiol-ene PEG and acrylate-based PEG hydrogels for cartilage development.
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DOI：
10.1016/j.biomaterials.2013.09.020
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发表时间：
2013-12
期刊：
影响因子：
14
通讯作者：
Bryant SJ
中科院分区：
文献类型：
--
作者：
Roberts JJ;Bryant SJ
在设计用于组织再生的水凝胶时，聚合机制和网络结构的差异有可能影响细胞行为。通过自由基光聚合丙烯酸酯（链式生长）和降冰片烯（阶梯生长）形成聚乙二醇水凝胶，研究水凝胶体系（聚合机理和网络结构）对工程组织发育的影响。将牛软骨细胞包裹在水凝胶中，在自由肿胀或动态压缩载荷下培养。在丙烯酸酯体系中，包封后的软骨细胞表现出高水平的细胞内ROS，同时水凝胶压缩模量降低，细胞在压缩应变下变形的变异性更高；在巯基-降冰片烯体系中未观察到的结果。长期来看，丙烯酸酯体系中磺化糖胺聚糖和总胶原的数量较多，但质量与增生性软骨相似，聚集蛋白、胶原I、II和X染色阳性，胶原分解代谢阳性。巯基-降冰片烯系统导致透明样软骨生成，特别是在机械负荷下，聚集蛋白和胶原蛋白II染色阳性，胶原蛋白I和X染色和胶原分解代谢染色最小。本研究结果证实了聚合机制和网络结构对工程软骨的质量有长期影响，特别是在机械载荷下。
When designing hydrogels for tissue regeneration, differences in polymerization mechanism and network structure have the potential to impact cellular behavior. Poly(ethylene glycol) hydrogels were formed by free-radical photopolymerization of acrylates (chain-growth) or thiol-norbornenes (step-growth) to investigate the impact of hydrogel system (polymerization mechanism and network structure) on the development of engineered tissue. Bovine chondrocytes were encapsulated in hydrogels and cultured under free swelling or dynamic compressive loading. In the acrylate system immediately after encapsulation chondrocytes exhibited high levels of intracellular ROS concomitant with a reduction in hydrogel compressive modulus and higher variability in cell deformation upon compressive strain; findings that were not observed in the thiol-norbornene system. Long-term the quantity of sulfated glycosaminoglycans and total collagen was greater in the acrylate system, but the quality resembled that of hypertrophic cartilage with positive staining for aggrecan, collagens I, II, and X and collagen catabolism. The thiol-norbornene system led to hyaline-like cartilage production especially under mechanical loading with positive staining for aggrecan and collagen II and minimal staining for collagens I and X and collagen catabolism. Findings from this study confirm that the polymerization mechanism and network structure have long-term effects on the quality of engineered cartilage, especially under mechanical loading.