Proteome profile and lentiviral transduction of cultured honey bee (Apis mellifera L.) cells
Proteome profile and lentiviral transduction of cultured honey bee (Apis mellifera L.) cells
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DOI：
10.1111/j.1365-2583.2010.01022.x
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发表时间：
2010-10-01
影响因子：
2.6
通讯作者：
Foster, L. J.
中科院分区：
文献类型：
--
作者：
Chan, M. M. Y.;Choi, S. Y. C.;Foster, L. J.
蜜蜂（Apis mellifera L.）作为传粉者在农业中起着至关重要的作用，是社会行为和免疫的模式生物。缺乏永生化细胞系和将重组DNA可靠地引入原代细胞的方法阻碍了对这种生物的细胞和分子研究。我们在此证明了一种绿色荧光蛋白基因通过慢病毒转导到培养的胚胎细胞中的表达。这种方法的成功表明，病毒转导可以用来传递组成活性癌基因，以使蜜蜂细胞永生。我们能够在几个月的低温储存后成功地恢复细胞，我们展示了新鲜收集的和培养的胚胎细胞之间的蛋白质组变化。
Honey bees (Apis mellifera L.) play a vital role in agriculture as pollinators, and serve as model organisms of social behaviour and immunity. The lack of both immortalized cell lines and methods to introduce recombinant DNA reliably into primary cells hinders cellular and molecular studies in this organism. We hereby demonstrate the expression of a GFP gene delivered by lentivirus transduction to cultured embryonic cells. The success of this approach indicates that viral transduction could be used to deliver constitutively active oncogenes in order to immortalize honey bee cells. We were able to revive cells successfully after several months of cryogenic storage and we show how the proteome varies between freshly collected and cultured embryonic cells.