Cutaneous water loss and covalently bound lipids of the stratum corneum in nestling house sparrows (Passer domesticus L.) from desert and mesic habitats
Cutaneous water loss and covalently bound lipids of the stratum corneum in nestling house sparrows (Passer domesticus L.) from desert and mesic habitats
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DOI：
10.1242/jeb.064972
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发表时间：
2012-04
影响因子：
2.8
通讯作者：
Michelle E. Clement;A. Muñoz-Garcia;Joseph B. Williams
中科院分区：
文献类型：
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作者：
Michelle E. Clement;A. Muñoz-Garcia;Joseph B. Williams
摘要 角质层 (SC) 是鸟类和哺乳动物表皮的外层，其脂质为水蒸气通过皮肤扩散提供了屏障。鸟类的 SC 由扁平的死细胞（称为角质细胞）和两个脂质区室组成：细胞间基质和附着在角质细胞外表面的单层共价结合脂质 (CBL)。我们之前发现两类鞘脂，神经酰胺和脑苷脂，与麻雀（Passer Domesticus L.）皮下角质层细胞共价结合；这些脂质与皮肤失水（CWL）有关。在这项研究中，我们收集了来自俄亥俄州的成年麻雀和雏鸟以及来自沙特阿拉伯的雏鸟，让它们适应高湿度或低湿度，并测量了它们的 CWL 率。我们还测量了来自俄亥俄州和沙特阿拉伯的雏鸟自然种群的 CWL，从雏鸟 2 天大直至长出羽毛为止。然后我们使用薄层色谱法评估了麻雀 SC 的 CBL 组成。我们发现成年麻雀的 SC 中 CBL 的多样性比之前描述的要高。在个体发育过程中，雏鸟的 CBL 数量增加，并且根据其栖息地的不同，其 CBL 的发育也有所不同。让雏鸟适应不同的湿度条件并没有改变 CBL 的个体发育，这表明这些脂质代表了 SC 组织的基本组成部分，不会对短期环境变化做出反应。
SUMMARY Lipids of the stratum corneum (SC), the outer layer of the epidermis of birds and mammals, provide a barrier to water vapor diffusion through the skin. The SC of birds consists of flat dead cells, called corneocytes, and two lipid compartments: an intercellular matrix and a monolayer of covalently bound lipids (CBLs) attached to the outer surface of the corneocytes. We previously found two classes of sphingolipids, ceramides and cerebrosides, covalently bound to corneocytes in the SC of house sparrows (Passer domesticus L.); these lipids were associated with cutaneous water loss (CWL). In this study, we collected adult and nestling house sparrows from Ohio and nestlings from Saudi Arabia, acclimated them to either high or low humidity, and measured their rates of CWL. We also measured CWL for natural populations of nestlings from Ohio and Saudi Arabia, beginning when chicks were 2 days old until they fledged. We then evaluated the composition of the CBLs of the SC of sparrows using thin layer chromatography. We found that adult house sparrows had a greater diversity of CBLs in their SC than previously described. During ontogeny, nestling sparrows increased the amount of CBLs and developed their CBLs differently, depending on their habitat. Acclimating nestlings to different humidity regimes did not alter the ontogeny of the CBLs, suggesting that these lipids represent a fundamental component of SC organization that does not respond to short-term environmental change.