Development of a novel method combining HPLC fingerprint and multi-ingredients quantitative analysis for quality evaluation of traditional chinese medicine preparation
Development of a novel method combining HPLC fingerprint and multi-ingredients quantitative analysis for quality evaluation of traditional chinese medicine preparation
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DOI：
10.1016/j.talanta.2011.04.059
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发表时间：
2011-08-15
期刊：
影响因子：
6.1
通讯作者：
Wu, Xiao-Wen
中科院分区：
文献类型：
--
作者：
Yang, Dong-Zhi;An, Ti Qiang;Wu, Xiao-Wen
采用高效液相色谱（HPLC）指纹图谱与多活性成分同时定量分析相结合的方法，对双黄连口服液进行了质量评价。采用指纹图谱分析法，选取45个共有峰，对不同厂家生产的双黄连口服液制剂进行相似性评价。此外，还对绿原酸、咖啡酸、芦丁、连翘酯苷、黄芩苷、木犀草苷、芹菜素、黄芩素和汉黄芩素等11种标记物进行了定量分析。所得数据的统计分析表明，我们的方法已达到预期的线性，精密度和准确度。最后对国内不同厂家生产的SHL口服液中这11种标志物的含量进行了测定。结果表明，HPLC指纹图谱与多组分同时定量相结合，为双黄连口服液制剂的质量评价提供了一种有效、可靠的方法。(C)2011 Elsevier B.V.保留所有权利。
A novel method combining high performance liquid chromatography (HPLC) fingerprint and simultanous quantitative analysis of multiple acitve components was developed and validated for quality evaluation of one type of traditional Chinese medicine preparations: Shuang-huang-lian (SHL) oral liquid formulation. For fingerprint analysis, 45 peaks were selected as the common peaks to evaluate the similarities among several different SHL oral liquid preparations collected from manufacturers. Additionally, simultanous quantification of eleven markers, including chlorogenic acid, caffeic acid, rutin, forsythiaside, scutellarin, baicalin, forsythin, luteoloside, apigenin, baicalein and wogonin, was performed. Statistical analysis of the obtained data demonstrated that our method has achieved desired linearity, precision and accuracy. Finally, concentrations of these eleven markers in SHL oral liquid prepared by different manufacturers in China were determined. These results demonstrated that the combination of HPLC chromatographic fingerprint and simultaneous quantification of multi-ingredients offers an efficient and reliable approach for quality evaluation of SHL oral liquid preparations. (C) 2011 Elsevier B.V. All rights reserved.