Effect of bilirubin in ischemia/reperfusion injury on rat small intestine
Effect of bilirubin in ischemia/reperfusion injury on rat small intestine
复制标题
DOI：
10.1053/jpsu.2001.28816
复制
发表时间：
2001-12-01
影响因子：
2.4
通讯作者：
Kale, N
中科院分区：
文献类型：
--
作者：
Ceran, C;Sönmez, K;Kale, N
目的：本研究的目的是确定胆红素对大鼠实验性小肠缺血/再灌注（I/R）损伤的影响。方法，30只大鼠分为5组（n = 6）。在S组中，通过颈静脉输注生理盐水，在B组中，通过颈静脉输注胆红素20mg/kg，无需额外的操作。 S-IR组、生理盐水组、B-1-IR组和B-2-IR组分别输注10 mg/kg/h和20 mg/kg/h胆红素2小时。在这些组中，输注后通过闭塞肠系膜上动脉 45 分钟然后再灌注 1 小时来进行 I/R 程序。再灌注后，切除小肠进行组织病理学和丙二醛（MDA）评估。粘膜损伤评分为0～5级，对丙二醛水平和组织病理学分级进行统计分析。结果：S-IR组粘膜损伤较重（4～5级），B-1-IR组粘膜损伤较轻（0～3级），B-2-IR组无粘膜损伤（0级）。 S-IR 组的评分在统计上高于 B-1-IR 和 B-2-IR 组 (P < .05)。 S-IR 组的组织 MDA 水平显着高于 B-1-IR 和 B-2-IR 组 (U = 36, P < .05)。胆红素水平与 MDA 水平呈负相关 (r = -0.94)。结论：胆红素可有效预防大鼠肠道 I/R 损伤。这一观察结果与胆红素作为抗氧化剂、在身体防御中发挥作用的假设是一致的。 WB 版权所有 (C) 2001。桑德斯公司。
Purpose: The aim of this study was to determine the effects of bilirubin in experimental small intestinal ischemia/reperfusion (I/R) injury in rats.Methods, Thirty rats were divided into 5 groups (n = 6). In group S, saline and in group B, bilirubin, 20 mg/kg were infused via the jugular vein without an additional procedure. In groups S-IR, saline, B-1-IR and B-2-IR, 10 and 20 mg/kg/h of bilirubin were infused for 2 hours, respectively. In these groups, an I/R procedure was done after infusions by occluding the superior mesenteric artery for 45 minutes followed by 1 hour of reperfusion. After reperfusion, the small intestines were resected for histopathologic and malondialdehyde (MDA) assessments. Mucosal lesions were scored between 0 and 5. Malondialdehyde levels and histopathologic grades were analyzed statistically.Results: Mucosal injury was severe in S-IR (grade 4 to 5), mild in B-1-IR (grade 0 to 3) and none in B-2-IR group (grade 0). Grades of group S-IR were higher than those of B-1-IR and B-2-IR statistically (P < .05). Tissue MDA levels of the S-IR group were significantly higher than those of B-1-IR and B-2-IR groups (U = 36, P < .05). Bilirubin levels correlated inversely with MDA levels (r = -0.94).Conclusions: Bilirubin effectively prevents intestinal I/R injury in rat. This observation is consistent with the hypotheses regarding bilirubin as an antioxidant, having a role in the body defense. Copyright (C) 2001 by WB. Saunders Company.