Expression of Annexin A5 Is Associated With Higher Tumor Stage and Poor Prognosis in Colorectal Adenocarcinomas
Expression of Annexin A5 Is Associated With Higher Tumor Stage and Poor Prognosis in Colorectal Adenocarcinomas
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膜联蛋白 a5 的表达与结直肠腺癌的较高肿瘤分期和不良预后相关
DOI：
10.1097/mcg.0b013e31819cc731
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发表时间：
2009-10-01
影响因子：
2.9
通讯作者：
Sun, Shu-Han
中科院分区：
文献类型：
--
作者：
Xue, Geng;Hao, Li-Qiang;Sun, Shu-Han
目的：了解膜联蛋白A5(ANXA5)在肿瘤分期及其临床转归中的作用。背景：ANXA5是一种钙结合蛋白，与多种肿瘤的发生有关。然而，ANXA5在结直肠癌中的作用尚不清楚。研究：我们研究了ANXA5在结直肠腺癌中的表达。这项研究包括207名连续的散发性结直肠癌患者。通过手术切除获得配对的大肠组织标本和相应的非恶性组织。采用实时定量逆转录聚合酶链式反应(RT-PCR)和免疫组织化学方法检测各组织中ANXA5基因和蛋白的表达。结果：实时定量逆转录聚合酶链式反应显示肿瘤组织中ANXASmRNA表达上调(P<；0.001)。免疫组织化学检测显示，40.58%(84/207)的肿瘤有ANTXA5高表达。单因素分析显示，ANXA5的表达与乳腺癌的PT分期(P=0.008)、肝转移(P=0.024)、病理分期(P=0.015)、Dukes‘s分期(P=0.017)、复发(P=0.024)、癌相关死亡(P=0.028)、无复发概率(P=0.003)和总生存率(P=0.005)相关。多因素分析显示，ANXA5的表达与无复发概率(P=0.039和P=0.048)和总生存期(P=0.012和P=0.021)显著相关，与PT分期和PN分期无关。结论：ANXA5的表达可能与结直肠癌的分期和临床预后有关。因此，ANXA5可作为肿瘤进展的预后标记物。
Goals: To gain an insight into the putative role of annexin A5 (ANXA5) in the tumor stage and its clinical outcome.Background: ANXA5 is a calcium-binding protein, which has been implicated in the carcinogenesis of several carcinomas. However, the role of ANXA5 in colorectal cancer (CRC) is unclear.Study: We investigated the expression of ANXA5 in colorectal adenocarcinoma. This study included 207 consecutive patients with sporadic CRC. Paired colorectal tissue samples and corresponding nonmalignant tissues were obtained by surgical resection. ANXA5 mRNA and protein expression in each tissue were assessed by real-time reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical staining. Data were statistically correlated with pathologic parameters and clinical outcome.Results: Real-time reverse transcriptase polymerase chain reaction showed that there is an up-regulation in the mRNA level of ANXAS in tumors (P < 0.001). lmmunohistochemical study revealed that high ANTXA5 expression was present in 40.58% (84 of 207) of tumors. Univariate analysis showed increased ANXA5 expression correlated with pT stage (P = 0.008), liver metastasis (P = 0.024), pathologic tumor-node-metastasis stage (P = 0.015), Dukes' stage (P = 0.017), recurrence (P = 0.024), cancer-related death (P = 0.028), recurrence-free probability (P = 0.003), and overall survival (P = 0.005). Multivariate analysis showed that ANXAS expression and liver metastasis significantly correlated with recurrence-free probability (P = 0.039 and P = 0.048, respectively) and overall survival (P = 0.012 and P = 0.021, respectively) independent of pT stage and pN stage.Conclusions: From these findings ANXA5 expression seems to be related to the tumor stage and clinical outcome of CRC. Thus ANXA5 could serve as a prognostic marker for tumor progression.