Molecular imaging assessment of periodontitis lesions in an experimental mouse model
Molecular imaging assessment of periodontitis lesions in an experimental mouse model
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DOI：
10.1007/s00784-018-2510-2
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发表时间：
2019-02-01
影响因子：
3.4
通讯作者：
Takashiba, Shogo
中科院分区：
文献类型：
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作者：
Ideguchi, Hidetaka;Yamashiro, Keisuke;Takashiba, Shogo
材料与方法将12只雌性小鼠随机分为对照组（n = 4）、牙周炎模型组（n = 4）、牙龈卟啉单胞菌（Porphyromonas gingivalis，Pg）组（n = 4）、牙周炎模型组（n = 4）和牙周炎模型组（n = 4）。Pg + GA组（n = 4）。所有小鼠均给予髓过氧化物酶（MPO）活性特异性发光探针，并在第14天使用电荷耦合器件相机进行观察。使用软件对所有小鼠进行图像分析以确定炎症的信号强度。此外，对每组3只小鼠进行牙周炎和牙周炎部位骨吸收的组织学和放射学评价，以及定量酶联免疫吸附测定（ELISA）。结果Pg组血清抗牙龈卟啉单胞菌IgG抗体水平显著高于Pg + GA组。组织学分析表明，破骨细胞和中性粒细胞的数量在Pg + GA中显着低于Pg组。Micro-CT图像分析表明Pg和Pg + GA组之间的骨吸收没有差异。MPO活性的信号强度检测到完整的颅面图像，而且，强信号强度是专门定位在牙周炎的网站在离体腭，与group-wise differences. ConclusionsMolecular imaging analysis based on MPO activity showed high sensitivity of detection of牙周炎in mice. Clinical relevance在小鼠的牙周炎的检测。
ObjectiveWe aimed to evaluate molecular imaging as a novel diagnostic tool for mice periodontitis model induced by ligature and Porphyromonas gingivalis (Pg) inoculation.Materials and methodsTwelve female mice were assigned to the following groups: no treatment as control group (n=4); periodontitis group induced by ligature and Pg as Pg group (n=4); and Pg group treated with glycyrrhizinic acid (GA) as Pg + GA group (n=4). All mice were administered a myeloperoxidase (MPO) activity-specific luminescent probe and observed using a charge-coupled device camera on day 14. Image analysis on all mice was conducted using software to determine the signal intensity of inflammation. Additionally, histological and radiographic evaluation for periodontal inflammation and bone resorption at the site of periodontitis, and quantitative enzyme-linked immunosorbent assay (ELISA) were conducted on three mice for each group. Each experiment was performed three times.ResultsLevels of serum IgG antibody against P. gingivalis were significantly higher in the Pg than in the Pg + GA group. Histological analyses indicated that the number of osteoclasts and neutrophils were significantly lower in the Pg + GA than in the Pg group. Micro-CT image analysis indicated no difference in bone resorption between the Pg and Pg + GA groups. The signal intensity of MPO activity was detected on the complete craniofacial image; moreover, strong signal intensity was localized specifically at the periodontitis site in the ex vivo palate, with group-wise differences.ConclusionsMolecular imaging analysis based on MPO activity showed high sensitivity of detection of periodontal inflammation in mice.Clinical relevanceMolecular imaging analysis based on MPO activity has potential as a diagnostic tool for periodontitis.