Lysis of small cell carcinoma of the lung tumor cell lines by gamma interferon-activated allogeneic peripheral blood mononuclear cells: abrogation of killing by pretreatment of tumor cells with gamma interferon.
Lysis of small cell carcinoma of the lung tumor cell lines by gamma interferon-activated allogeneic peripheral blood mononuclear cells: abrogation of killing by pretreatment of tumor cells with gamma interferon.
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γ干扰素激活的同种异体外周血单核细胞裂解肺肿瘤细胞系的小细胞癌：用γ干扰素预处理肿瘤细胞取消杀伤作用。
DOI：
10.1007/bf00200035
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Fanger,MW
中科院分区：
文献类型：
--
作者：
Ball,ED;Nichols,KE;Pettengill,OS;Sorenson,GD;Fanger,MW
干扰素已被证明可以增强非特异性细胞毒性单核细胞裂解部分而非全部肿瘤细胞的能力。我们研究了重组人γ干扰素（rIFNγ）对来自连续培养的小细胞肺癌（SCCL）系的肿瘤靶细胞的细胞介导的细胞溶解作用的影响。来自 SCCL 系 DMS 44、53、79、92 和 406 的细胞用 51 Cr 标记，并与正常和 rIFNγ 刺激的外周血单核细胞在 37℃下孵育 18 小时，并通过测量 51 Cr 释放来估计肿瘤细胞裂解。尽管来自某些 SCCL 系的细胞是细胞介导的细胞毒性的良好靶标，但不同 SCCL 系之间对裂解的敏感性是异质的。平均而言，DMS 406 和 79 被最大程度地裂解，而 DMS 44、53 和 92 显示出对对照或 rIFNγ 刺激的效应细胞裂解的敏感性较低。此外，虽然rIFNγ预处理增加了来自几个不同供体的正常外周血单核细胞的细胞溶解能力，但用rIFNγ预孵育肿瘤细胞系导致对照和IFN激活效应细胞介导的细胞溶解抑制。这些发现表明，虽然 rIFNγ 可能增强 SCCL 肿瘤细胞的细胞介导的裂解，但它也可能降低裂解的敏感性。
Interferon has been shown to enhance the ability of nonspecific cytotoxic mononuclear cells to lyse some, but not all, tumor cells. We have examined the effect of recombinant human gamma interferon (rIFNγ) on the cell-mediated cytolysis of tumor target cells derived from continuously cultured lines of small cell carcinoma of the lung (SCCL). Cells from the SCCL lines DMS 44, 53, 79, 92, and 406 were labeled with51Cr and incubated with normal and rIFNγ-stimulated peripheral blood mononuclear cells for 18 h at 37 °C and tumor cell lysis estimated by measuring51Cr release. Although cells from certain SCCL lines were good targets for cell mediated cytotoxicity, susceptibility to lysis was heterogenous among the different SCCL lines. DMS 406 and 79 were, on average, maximally lysed, while DMS 44, 53, and 92 showed less susceptibility to lysis by either control or rIFNγ-stimulated effector cells. In addition, although pretreatment with rIFNγ increased the cytolytic capacity of normal peripheral blood mononuclear cells from several different donors, preincubation of the tumor cell lines with rIFNγ resulted in inhibition of cytolysis mediated by both control and IFN-activated effector cells. These findings suggest that although rIFNγ may enhance cell-mediated lysis of SCCL tumor cells, it may also decrease susceptibility to lysis.