In vivo dynamic human retinal blood flow imaging using ultra-high-speed spectral domain optical coherence tomography.
In vivo dynamic human retinal blood flow imaging using ultra-high-speed spectral domain optical coherence tomography.
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DOI：
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发表时间：
2003
期刊：
影响因子：
3.8
通讯作者：
B. White;M. Pierce;N. Nassif;B. Cense;B. Park;G. Tearney;B. Bouma;Teresa Chen;J. D. de Boer
中科院分区：
文献类型：
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作者：
B. White;M. Pierce;N. Nassif;B. Cense;B. Park;G. Tearney;B. Bouma;Teresa Chen;J. D. de Boer
超高速光谱域光学多普勒层析成像(SD-ODT)系统被用来以每秒29,000个深度剖面(A线)采集活体人类视网膜内的血流图像，并且数据采集超过99%的测量时间。对系统的相位稳定性进行了分析，并给出了精确确定双向多普勒频移的图像处理算法。在3.28秒的时间段内，以每秒29帧、每帧1000条A线的速度采集人类视网膜血流的电影，显示了动脉-静脉对中血管边界的准确确定和随时间变化的双向血流动力学。超高速SD-ODT系统可以可视化视网膜血流的脉动性，检测脉络膜和视网膜毛细血管内的血流，并提供有关心脏周期的信息。综上所述，在眼睛暴露水平低于600微瓦的情况下，视网膜血流动力学的准确视频速率成像被展示出来。
An ultra-high-speed spectral domain optical Doppler tomography (SD-ODT) system is used to acquire images of blood flow in a human retina in vivo, at 29,000 depth profiles (A-lines) per second and with data acquisition over 99% of the measurement time. The phase stability of the system is examined and image processing algorithms are presented that allow accurate determination of bi-directional Doppler shifts. Movies are presented of human retinal flow acquired at 29 frames per second with 1000 A-lines per frame over a time period of 3.28 seconds, showing accurate determination of vessel boundaries and time-dependent bi-directional flow dynamics in artery-vein pairs. The ultra-high-speed SD-ODT system allows visualization of the pulsatile nature of retinal blood flow, detects blood flow within the choroid and retinal capillaries, and provides information on the cardiac cycle. In summary, accurate video rate imaging of retinal blood flow dynamics is demonstrated at ocular exposure levels below 600 microW.