Molecular Biology and Genetic Engineering in Nitrogen Fixation
Molecular Biology and Genetic Engineering in Nitrogen Fixation
复制标题
DOI：
10.1007/978-1-61779-194-9_6
复制
发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Dos Santos, Patricia C.
中科院分区：
文献类型：
--
作者：
Dos Santos, Patricia C.
生物固氮是一个复杂而严格调控的过程，仅限于一组被称为固氮生物的原核生物。在已被充分研究的固氮菌中，棕色固氮菌因其方便好氧生长、高水平的固氮酶表达和遗传易处理性而被研究得最好。本章介绍了A. vinelandii已被用作推进固氮生物合成、机制和调控研究的有价值的工具。
Biological nitrogen fixation is a complex and tightly regulated process limited to a group of prokaryotic species known as diazotrophs. Among well-studied diazotrophs, Azotobacter vinelandii is the best studied for its convenience of aerobic growth, its high levels of nitrogenase expression, and its genetic tractability. This chapter includes protocols and strategies in the molecular biology and genetic engineering of A. vinelandii that have been used as valuable tools for advancing studies on the biosynthesis, mechanism, and regulation of nitrogen fixation.