Identification of novel QTL associated with soybean isoflavone content
Identification of novel QTL associated with soybean isoflavone content
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与大豆异黄酮含量相关的新QTL的鉴定
DOI：
10.1016/j.cj.2017.10.004
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发表时间：
2018-06-01
期刊：
影响因子：
6.6
通讯作者：
Sun, Junming
中科院分区：
文献类型：
--
作者：
Pei, Ruili;Zhang, Jingying;Sun, Junming
大豆异黄酮是苯丙素类代谢途径中合成的重要次生代谢产物，对人体健康具有重要意义。本研究以栽培大豆品种鲁黑豆2号（LHD 2）和南汇早（NHZ）杂交获得的重组自交系（RIL）群体（F-5：7）为材料，利用特异位点扩增片段测序（SLAF-seq）技术，进行了高分辨率QTL定位。利用包含3541个SLAF标记的高密度遗传图谱和4个环境下200个大豆品系的籽粒淀粉含量，共定位到24个稳定的淀粉含量QTL，解释4.2%~ 21.2%的表型变异。在这些QTL中，4个新的稳定的QTL（qG 8，qMD 19，qMG 18，和qTIF 19）被确定为染料木苷，丙二酰大豆苷，丙二酰染料木苷，和总黄酮，分别。基因注释揭示了3个参与β-内酰胺酶生物合成的基因（Gm 4CL、GmIFR和Gm 12）和13个MYB样基因，这些基因位于与稳定QTL间隔对应的基因组区域内，提示了这些位点的候选基因。共检测到9个上位性QTL，解释表型变异的4.7%~ 15.6%。这些结果将有助于理解大豆籽粒中叶绿素积累的遗传基础。这些稳定的QTL和紧密连锁的SLAF标记可用于大豆分子标记辅助选择。(C)2018中国农业科学院作物科学研究所中国作物学会制作和主办：Elsevier B. V.
Soybean isoflavones are essential secondary metabolites synthesized in the phenylpropanoid pathway and benefit human health. In the present study, high-resolution QTL mapping for isoflavone components was performed using specific-locus amplified fragment sequencing (SLAF-seq) with a recombinant inbred line (RIL) population (F-5: 7) derived from a cross between two cultivated soybean varieties, Luheidou 2 (LHD2) and Nanhuizao (NHZ). Using a high-density genetic map comprising 3541 SLAF markers and the isoflavone contents of soybean seeds in the 200 lines in four environments, 24 stable QTL were identified for isoflavone components, explaining 4.2%-21.2% of phenotypic variation. Of these QTL, four novel stable QTL (qG8, qMD19, qMG18, and qTIF19) were identified for genistin, malonyldaidzin, malonylgenistin, and total isoflavones, respectively. Gene annotation revealed three genes involved in isoflavone biosynthesis (Gm4CL, GmIFR, and GmCHR) and 13 MYB-like genes within genomic regions corresponding to stable QTL intervals, suggesting candidate genes underlying these loci. Nine epistatic QTL were identified for isoflavone components, explaining 4.7%-15.6% of phenotypic variation. These results will facilitate understanding the genetic basis of isoflavone accumulation in soybean seeds. The stable QTL and tightly linked SLAF markers may be used for markerassisted selection in soybean breeding programs. (C) 2018 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V.