Phycobilisome-associated glycoproteins in the cyanobacterium Anacystis nidulans R 2.
Phycobilisome-associated glycoproteins in the cyanobacterium Anacystis nidulans R 2.
复制标题
蓝藻 Anacystis nidulans R 2 中的藻胆体相关糖蛋白。
DOI：
10.1016/0014-5793(87)80147-3
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发表时间：
1987
期刊：
影响因子：
3.5
通讯作者：
Sherman,LA
中科院分区：
文献类型：
--
作者：
Riethman,HC;Mawhinney,TP;Sherman,LA
在聚丙烯酰胺凝胶上分离分离的 Anacystis nidulansR 2 藻胆体的亚基并转移到硝酸纤维素上后，发现四种藻胆体相关蛋白与凝集素伴刀豆球蛋白 A 发生特异性反应。伴刀豆球蛋白 A 反应性藻胆体成分具有与藻胆体的定向、组装和膜附着相关的功能。对总分离藻胆体材料的化学分析表明存在葡萄糖（约 1.5% 重量）和 N-乙酰半乳糖胺（0.15% 重量），这与我们的建议一致，即刀豆球蛋白 A 反应性多肽含有共价连接的含葡萄糖多糖。
Four phycobilisome-associated proteins were found to be specifically reactive with the lectin concanavalin A after subunits of isolatedAnacystis nidulansR 2 phycobilisomes were separated on polyacrylamide gels and transferred onto nitrocellulose. The concanavalin A-reactive phycobilisome components have proposed functions related to the orientation, assembly, and membrane attachment of the phycobilisome. Chemical analysis of the total isolated phycobilisome material indicated the presence of glucose (approx. 1.5% by wt) andN-acetylgalactosamine (0.15% by wt), consistent with our proposal that the concanavalin A-reactive polypeptides contain covalently linked, glucose-containing polysaccharides.